LOCUS       Exported                5563 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pcDNA1-hTNFR55-E, complete sequence.
ACCESSION   LT727190
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Mammalian expression vector pcDNA1-hTNFR55-E"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4340."
                     /db_xref="taxon:1944922"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /direction=LEFT
                     /label=polyoma ori
                     /note="polyoma ori"
     polyA_signal    1006..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1066..1162)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      1066..1162
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(1163..1561)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1412..1432)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1562..1628)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     intron          1562..1627
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1629..1703)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL87720.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     promoter        1711..1729
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      1711..1727
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     misc_feature    complement(1871..1909)
                     /label=E-tag
                     /note="E-tag"
     CDS             complement(1916..3178)
                     /codon_start=1
                     /note="unnamed protein product; hTNFR55 mature sequence"
                     /protein_id="SJL87721.1"
                     /translation="MVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPG
                     PGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYR
                     HYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECT
                     KLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKS
                     TPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAP
                     RREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPL
                     RWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDM
                     DLLGCLEDIEEALCGPAALPPA"
     CDS             complement(3179..3265)
                     /codon_start=1
                     /note="unnamed protein product; hTNFR55 signal sequence"
                     /protein_id="SJL87722.1"
                     /translation="MGLSTVPDLLLPLVLLELLVGIYPSGVIG"
     promoter        complement(3376..3394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(3378..3394)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      complement(3439..4022)
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     promoter        complement(3439..3642)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3643..4022
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(4177..4379)
                     /label=supF
                     /note="supF"
     tRNA            complement(4181..4360)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     rep_origin      4503..4961
                     /label=M13 ori
                     /note="M13 ori"
     rep_origin      complement(4980..5212)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"