LOCUS       Exported                7657 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  UNVERIFIED: Cloning vector pcDNA-mBeta-cat-Tcf3, complete sequence.
ACCESSION   KM189193
VERSION     .
KEYWORDS    UNVERIFIED.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     MYCN Integrative Omics Reveal Context Dependent Transcriptional 
            Repressor/Activator Functions and Discover Potential Therapeutic 
            Targets
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Relaix F, Murphy P, Avner P, Duffy DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2014) Systems Biology Ireland, University College 
            Dublin, Belfield, Dublin, Leinster 0004, Ireland
REFERENCE   3  (bases 1 to 7657)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     GenBank staff is unable to verify sequence and/or annotation 
            provided by the submitter.
            ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Cloning vector pcDNA-mBeta-cat-Tcf3"
                     /mol_type="other DNA"
                     /label=derived from Mus musculus
                     /note="derived from Mus musculus"
                     /db_xref="taxon:1636445"
     CDS             complement(827..1206)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5-AACGTT-3 (Sato et al., 1996)"
     promoter        complement(1391..1495)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1761..2140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2141..2344
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2389..2407
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2431..2440
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2437..4470
                     /codon_start=1
                     /product="truncated beta-catenin Tcf7l1 fusion protein"
                     /label=truncated beta-catenin Tcf7l1 fusion protein
                     /note="FLAG tagged"
                     /protein_id="AKJ66811.1"
                     /translation="MDYKDDDDKDPPISRGSEFTYAAAVLFRMSEDKPQDYKKRLSVEL
                     TSSLFRTEPMAWNETADLGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEH
                     EMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDLPGIDVDGIDKLDNES
                     ENQSSSSDSEAERRPQPARDAFQKPRDYFAEVRRPQDGAFFKGPAYPGYPFLMIPDLSS
                     PYLSNGPLSPGGARTYLQMKWPLLDVPSSATVKDTRSPSPAHLSNKVPVVQHPHHMHPL
                     TPLITYSNDHFSPASPPTHLSPEIDPKTGIPRPPHPSELSPYYPLSPGAVGQIPHPLGW
                     LVPQQGQPMYSLPPGGFRHPYPALAMNASMSSLVSSRFPHMVAPAHPGLPTSGIPHPAI
                     VSPIVKQEPAAPSLSPAVSAKSPVTVKKEEEKKPHVKKPLNAFMLYMKEMRAKVVAECT
                     LKESAAINQILGRKWHNPSREEQAKYYELARKERQLHAQLYLTWSARDNYGKKKKRKRE
                     KQLSQTQSQQQIQEAEGALASKSKKPCIQYLPPEKPCDSPASSHGSTLDSPATPSAALA
                     SPAAPAATHSEQAQPLSLTTKPEARAQLALHSAAFLSAKAAASNSSQMGSQPPLLSRPL
                     PLGSMPAALLTSPPTFPATLHAHQALPVLQAQPLSLVTKSAH"
     CDS             2440..2463
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4707..4725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(4736..4754)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4780..5004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5050..5478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5492..5821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5672..5807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6855..6976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7025..7041)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7049..7065
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7073..7103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7118..7139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7427..358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"