LOCUS       Exported                6188 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCDM92, complete sequence.
ACCESSION   LT727298
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6188)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Mammalian expression vector pCDM92"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3980."
                     /db_xref="taxon:1944915"
     rep_origin      25..157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..108)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(259..371)
                     /direction=LEFT
                     /label=polyoma ori
                     /note="polyoma ori"
     polyA_signal    1000..1134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1060..1156)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      1060..1156
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(1157..1555)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1406..1426)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1556..1622)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     CDS             complement(1623..1697)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL88023.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1728..1927)
                     /label=3 UTR of hIFNbeta
                     /note="3 UTR of hIFNbeta"
     CDS             complement(1928..2491)
                     /codon_start=1
                     /note="unnamed protein product; hIFNbeta"
                     /protein_id="SJL88024.1"
                     /translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQL
                     NGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETI
                     VENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCA
                     WTIVRVEILRNFYFINRLTGYLRN"
     promoter        complement(2527..2545)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2529..2545)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      complement(2590..3173)
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     promoter        complement(2590..2793)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2794..3173
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(3328..3530)
                     /label=supF
                     /note="supF"
     tRNA            complement(3332..3511)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     CDS             complement(4122..4982)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL88025.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4983..5087)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5605..5837)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"