LOCUS       Exported                6191 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCDM9, complete sequence.
ACCESSION   LT727197
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6191)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Mammalian expression vector pCDM9"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4364."
                     /db_xref="taxon:1944914"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     misc_feature    complement(262..1004)
                     /label=PY
                     /note="PY"
     rep_origin      complement(262..374)
                     /direction=LEFT
                     /label=PY-O
                     /note="PY-O"
     polyA_signal    1003..1137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1063..1159)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1063..1159
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    complement(1160..1558)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(1409..1429)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1559..1625)
                     /label=intron
                     /note="intron"
     CDS             complement(1626..1700)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL87739.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1731..1930)
                     /label=3 UTR of hIFNB
                     /note="3 UTR of hIFNB"
     CDS             complement(1931..2494)
                     /codon_start=1
                     /note="unnamed protein product; hIFNB"
                     /protein_id="SJL87740.1"
                     /translation="MTNKCLLQIALLLCFSTTALSMSYNLLGFLQRSSNFQCQKLLWQL
                     NGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETI
                     VENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCA
                     WTIVRVEILRNFYFINRLTGYLRN"
     promoter        complement(2530..2548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2532..2548)
                     /regulatory_class="promoter"
                     /note="T7"
     regulatory      complement(2593..3176)
                     /regulatory_class="promoter"
                     /note="hCMV"
     promoter        complement(2593..2796)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2797..3176
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(3331..3533)
                     /label=supF
                     /note="supF"
     tRNA            complement(3335..3514)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     CDS             complement(4125..4985)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87741.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4986..5090)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5608..5840)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"