LOCUS       Exported                5130 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCDM8hFasR, complete sequence.
ACCESSION   LT727441
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5130)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Mammalian expression vector pCDM8hFasR"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3118."
                     /db_xref="taxon:1944911"
     rep_origin      25..160
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(36..111)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 ori"
     rep_origin      complement(262..374)
                     /direction=LEFT
                     /label=polyoma ori
                     /note="polyoma ori"
     polyA_signal    1003..1137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1063..1159)
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     regulatory      1063..1159
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     misc_feature    complement(1160..1558)
                     /label=3 UTR of SV40 small t-antigen
                     /note="3 UTR of SV40 small t-antigen"
     CDS             complement(1409..1429)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    complement(1559..1625)
                     /label=intron of SV40 small t-antigen
                     /note="intron of SV40 small t-antigen"
     CDS             complement(1626..1700)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small 
                     t-antigen"
                     /protein_id="SJL88463.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     CDS             complement(1703..2710)
                     /codon_start=1
                     /note="unnamed protein product; hFAS"
                     /protein_id="SJL88464.1"
                     /translation="MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTVE
                     TQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRC
                     RLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNT
                     KCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAI
                     NLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNW
                     HQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV"
     misc_feature    complement(2711..2907)
                     /label=5 UTR of hFAS
                     /note="5 UTR of hFAS"
     promoter        complement(2943..2961)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(2945..2961)
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     regulatory      complement(3006..3589)
                     /regulatory_class="promoter"
                     /note="hCMV-IE promoter and enhancer"
     promoter        complement(3006..3209)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3210..3589
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    complement(3744..3946)
                     /label=supF
                     /note="supF"
     tRNA            complement(3748..3927)
                     /product="E. coli amber-suppressor tyrosine tRNA"
                     /label=supF
     rep_origin      4070..4528
                     /label=M13 ori
                     /note="M13 ori"
     rep_origin      complement(4547..4779)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"