| 载体基本信息 |
| 载体名称 |
pCD-RPyr4T |
载体长度 |
9065 bp |
| 载体类型 |
Suicide vector |
载体来源 |
Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR. |
pCD-RPyr4T 载体载体图谱
pCD-RPyr4T 载体载体序列
LOCUS Exported 9065 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Suicide vector pCD-RPyr4T, complete sequence.
ACCESSION KT176094
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9065)
AUTHORS Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
TITLE Novel strategies for genomic manipulation of Trichoderma reesei with
the purpose of strain engineering
JOURNAL Appl. Environ. Microbiol. (2015) In press
PUBMED 26150462
REFERENCE 2 (bases 1 to 9065)
AUTHORS Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien,
Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE 3 (bases 1 to 9065)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9065
/organism="Suicide vector pCD-RPyr4T"
/mol_type="other DNA"
/db_xref="taxon:1679964"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(7165..7753)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter complement(8938..9042)
/gene="bla"
/label=AmpR promoter
