LOCUS       Exported                5146 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-EYFP, complete sequence.
ACCESSION   KT176096
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 5146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Suicide vector pCD-EYFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1679961"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      544..553
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(2925..2941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2949..2965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2973..3003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3018..3039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3327..3915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4086..4946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4947..5051)
                     /gene="bla"
                     /label=AmpR promoter