This is an expression plasmid, which produces L1 and L2 of HPV33 in mammalian cells.
| 载体基本信息 | |||
| 载体名称 | pVITRO-HPV33 L1L2 | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 9205 bp | 载体类型 | Mammalian Expression |
| 筛选标记 | Neomycin (select with G418) | 拷贝数 | High Copy |
| 克隆方法 | Restriction Enzyme | 5引物 | GCCTCTTAGCGGTTCAAAGG |
| 3引物 | CAAAGGTATTTTCTAAACCCGTTTC | ||
pVITRO-HPV33 L1L2 载体载体图谱
pVITRO-HPV33 L1L2 载体载体序列
LOCUS Exported 9205 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Expresses HPV33 L1 and L2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pVITRO-HPV33 L1L2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9205)
AUTHORS Kwak K, Jiang R, Wang JW, Jagu S, Kirnbauer R, Roden RB
TITLE Impact of inhibitors and L2 antibodies upon the infectivity of
diverse alpha and beta human papillomavirus types.
JOURNAL PLoS One. 2014 May 9;9(5):e97232. doi: 10.1371/journal.pone.0097232.
eCollection 2014.
PUBMED 24816794
REFERENCE 2 (bases 1 to 9205)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
date: "2014-05-9"; volume: "9(5):e97232. doi"; pages: "
10.1371/journal.pone.0097232. eCollection 2014"
FEATURES Location/Qualifiers
source 1..9205
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(12..32)
/label=pBABE 3
/note="SV40 enhancer, reverse primer for pBABE vectors"
intron 521..1468
/label=mEF-1-alpha intron
/note="intron upstream of the start codon of mouse
EF-1-alpha"
polyA_signal 3048..3169
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3059..3078)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3113..3132
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 3357..3945
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3846..3865
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 4025..4328
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
misc_feature 7186..7630
/label=FMDV IRES
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 7663..7709
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 7701..7719
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 7729..8523
/codon_start=1
/gene="aph(3)-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7783..7802)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8393..8412
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8570..9142
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
