LOCUS       Exported                9670 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCC-ccdB, complete sequence.
ACCESSION   MG867731
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Spatial Interference Scale as a Determinant of Microbial Community 
            Assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   Lu T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2018) Bioengineering, University of Illinois at 
            Urbana-Champaign, 1304 W Springfield Ave, Urbana, IL 61801, USA
REFERENCE   3  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Cloning vector pCC-ccdB"
                     /mol_type="other DNA"
                     /db_xref="taxon:2302865"
     CDS             complement(337..1146)
                     /codon_start=1
                     /gene="luxR"
                     /product="LuxR"
                     /label=luxR
                     /protein_id="AXN93635.1"
                     /translation="MIYNTQNLRQTIGKDKEMGMKNINADDTYRIINKIKACRSNNDIN
                     QCLSDMTKMVHCEYYLLAIIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYS
                     NSNHSPINWNIFENNAVNKKSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKD
                     NYIDSLFLHACMNIPLIVPSLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDI
                     SKILGCSERTVTFHLTNAQMKLNTTNRCQSISKAILTGAIDCPYFKN"
     gene            complement(337..1146)
                     /gene="luxR"
                     /label=luxR
     regulatory      complement(1198..1253)
                     /regulatory_class="promoter"
                     /note="promoter PluxR"
     regulatory      1234..1292
                     /regulatory_class="promoter"
                     /note="promoter PluxI"
     CDS             1372..1632
                     /codon_start=1
                     /gene="ccdB"
                     /product="truncated CcdB"
                     /label=ccdB
                     /protein_id="AXN93634.1"
                     /translation="MFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDES
                     WRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            1372..1632
                     /gene="ccdB"
                     /label=ccdB
     regulatory      1647..1759
                     /regulatory_class="terminator"
                     /note="T1 terminator"
     promoter        1853..1871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1874..1930
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1973..1989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1997..2013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2021..2051)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2066..2087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             complement(2307..2966)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AXN93633.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(2307..2966)
                     /gene="cat"
                     /label=cat
     promoter        complement(2967..3069)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      3928..4542
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4618..4837
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4928..5683
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5686..5936
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6262..7437
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7437..8408
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    8481..8954
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    9214..9612
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    9630..9663
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."