LOCUS       Exported                3574 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCB301, complete sequence.
ACCESSION   AF139061
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     A mini binary vector series for plant transformation
  JOURNAL   Plant Mol. Biol. 40 (4), 711-717 (1999)
  PUBMED    10480394
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Xiang C, Han P, Lutziger I, Wang K, Oliver DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-1999) Botany, Iowa State University, Bessey Hall, 
            Ames, IA 50011, USA
REFERENCE   3  (bases 1 to 3574)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Binary vector pCB301"
                     /lab_host="Agrobacterium tumefaciens"
                     /mol_type="other DNA"
                     /label=plant transformation vector
                     /note="plant transformation vector"
                     /db_xref="taxon:98589"
     rep_origin      1..630
                     /label=RK2 oriV
                     /note="RK2 oriV"
     misc_feature    631..1636
                     /label=similar to nptIII
                     /note="similar to nptIII"
     CDS             842..1636
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1637..3129
                     /label=plasmid RK2 TrfA region
                     /note="plasmid RK2 TrfA region"
     CDS             1935..3083
                     /codon_start=1
                     /product="TrfA"
                     /label=TrfA
                     /protein_id="AAD43065.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    3130..3299
                     /label=T-DNA right border
                     /note="T-DNA right border"
     misc_feature    3230..3417
                     /label=multiple cloning site region
                     /note="multiple cloning site region"
     misc_feature    3235..3259
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    3300..3407
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     3324..3340
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3374..3390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3418..3574
                     /label=T-DNA left border
                     /note="T-DNA left border"
     misc_feature    3471..3495
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      3573..630
                     /label=oriV
                     /note="incP origin of replication"