LOCUS       Exported                4623 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Plant transformation vector pCB301 plasmid pXT1, complete sequence.
ACCESSION   JN029690
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Construction of Agrobacterium-mediated Cucumber Mosaic Virus 
            Infectious cDNA Clones and 2b Deletion Viral Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Tao X, Yao M, Hu Z, Wei Q, Feng Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2011) Department of Plant Pathology, Nanjing 
            Agricultural University, No.1 Weigang Street, Nanjing, Jiangsu 
            210095, P.R.China
REFERENCE   3  (bases 1 to 4623)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Plant transformation vector pCB301"
                     /plasmid="pXT1"
                     /lab_host="Agrobacterium tumefaciens"
                     /mol_type="other DNA"
                     /db_xref="taxon:1036807"
     regulatory      1..767
                     /regulatory_class="promoter"
                     /note="from gene 2x35S"
     promoter        722..767
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     misc_feature    768..800
                     /label=multiple cloning site region
                     /note="multiple cloning site region"
     ncRNA           800..887
                     /product="HDV-RZ"
                     /label=ribozyme
                     /note="from hepatitis delta virus"
                     /ncRNA_class="ribozyme"
     regulatory      888..1157
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      898..1150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1158..1327
                     /label=T-DNA right border (RB)
                     /note="T-DNA right border (RB)"
     misc_feature    1198..1222
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1374..2522)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2821..3615)
                     /codon_start=1
                     /gene="nptIII"
                     /product="neomycin phosphotransferase III nptIII"
                     /label=nptIII
                     /protein_id="AEH04539.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(2821..3615)
                     /gene="nptIII"
                     /label=nptIII
     rep_origin      3827..4458
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    4457..4613
                     /label=T-DNA left border (LB)
                     /note="T-DNA left border (LB)"
     misc_feature    4536..4560
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"