LOCUS       Exported                7758 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCB112-HPV11-L1, complete sequence.
ACCESSION   DQ191757
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson AL.
  TITLE     Evaluation of recombinant BCG expressing rotavirus VP6 as an 
            anti-rotavirus vaccine
  JOURNAL   Vaccine 25 (18), 3646-3657 (2007)
  PUBMED    17339069
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Pupa D.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-AUG-2005) Medical Virology, University of Cape Town, 
            Anzio Road, Observatory, Cape Town 7925, South Africa
REFERENCE   3  (bases 1 to 7758)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Shuttle vector pCB112-HPV11-L1"
                     /mol_type="other DNA"
                     /label=Mycobacterium-E. coli shuttle vector
                     /note="Mycobacterium-E. coli shuttle vector"
                     /db_xref="taxon:345408"
     regulatory      163..168
                     /regulatory_class="minus_35_signal"
                     /note="BCG hsp60 promoter"
     regulatory      186..191
                     /regulatory_class="minus_10_signal"
                     /note="BCG hsp60 promoter"
     misc_feature    198
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      364..370
                     /regulatory_class="ribosome_binding_site"
     CDS             382..1749
                     /codon_start=1
                     /product="BCG hsp60-lysA chimaera"
                     /label=BCG hsp60-lysA chimaera
                     /protein_id="ABB00292.1"
                     /translation="MAKTIADPDVNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQ
                     EYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTG
                     GELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGI
                     VQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSH
                     IGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPI
                     AELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRR
                     YVSVDGGMSDNIRTGLYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPTI
                     FGPAIWLRLPPPALLLFAVESLQHGRPSRCGSGARGNARLVLRRETVDDLLSLEVR"
     CDS             1877..2692
                     /codon_start=1
                     /product="aminoglycoside-3-phosphotransferase"
                     /function="kanamycin resistance"
                     /label=aminoglycoside-3-phosphotransferase
                     /protein_id="ABB00293.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2979..3766
                     /label=Ori E region derived from pUC19
                     /note="Ori E region derived from pUC19"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3771..5666
                     /note="Ori M region: mycobacterial origin of replication 
                     derived from pAL5000 presented in Genbank Accession Number 
                     M60875"
     regulatory      5873..5878
                     /regulatory_class="minus_35_signal"
                     /note="Mycobacterium leprae 18 kDa promoter"
     regulatory      5896..5901
                     /regulatory_class="minus_10_signal"
                     /note="Mycobacterium leprae 18 kDa promoter"
     misc_feature    5910
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      5960..5966
                     /regulatory_class="ribosome_binding_site"
     CDS             5975..7615
                     /codon_start=1
                     /product="BCG alpha antigen-HPV L1 chimaera"
                     /label=BCG alpha antigen-HPV L1 chimaera
                     /protein_id="ABB00294.1"
                     /translation="MATDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFGGP
                     MWRPSDSTVYVPPPNPVSKVVATDAYVKRTNIFYHASSSRLLAVGHPYYSIKKVNKTVV
                     PKVSGYQYRVFKVVLPDPNKFALPDSSLFDPTTQRLVWACTGLEVGRGQPLGVGVSGHP
                     LLNKYDDVENSGGYGGNPGQDNRVNVGMDYKQTQLCMVGCAPPLGEHWGKGTQCSNTSV
                     QNGDCPPLELITSVIQDGDMVDTGFGAMNFADLQTNKSDVPLDICGTVCKYPDYLQMAA
                     DPYGDRLFFYLRKEQMFARHFFNRAGTVGEPVPDDLLVKGGNNRSSVASSIYVHTPSGS
                     LVSSEAQLFNKPYWLQKAQGHNNGICWGNHLFVTVVDTTRSTNMTLCASVSKSATYTNS
                     DYKEYMRHVEEFDLQFIFQLCSITLSAEVMAYIHTMNPSVLEDWNFGLSPPPNGTLEDT
                     YRYVQSQAITCQKPTPEKEKQDPYKDMSFWEVNLKEKFSSELDQFPLGRKFLLQSGYRG
                     RTSARTGIKRPAVSKPSTAPKRKRTKTKK"
     terminator      7676..7722
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      7679..7722
                     /regulatory_class="terminator"
                     /note="rrnBT1"