LOCUS       Exported                7432 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pCB112-Edim6, complete sequence.
ACCESSION   DQ019611
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7432)
  AUTHORS   Dennehy M, Bourn W, Steele D, Williamson A-L.
  TITLE     Recombinant BCG expressing rotavirus VP6 partially protects mice 
            from rotavirus challenge
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7432)
  AUTHORS   Dennehy M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-2005) Virology, University of Cape Town, Anzio 
            Road Observatory, Cape Town, WP 7925, South Africa
REFERENCE   3  (bases 1 to 7432)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7432
                     /organism="Shuttle vector pCB112-Edim6"
                     /mol_type="other DNA"
                     /note="Rotavirus EDIM VP6 cloned into ApaI-ClaI sites of 
                     pCB112"
                     /db_xref="taxon:329805"
     regulatory      163..168
                     /regulatory_class="minus_35_signal"
                     /note="derived from hsp60 promoter"
     regulatory      186..191
                     /regulatory_class="minus_10_signal"
                     /note="derived from hsp60 promoter"
     misc_feature    198
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      364..370
                     /regulatory_class="ribosome_binding_site"
     CDS             382..1749
                     /codon_start=1
                     /product="BCG hsp60-lysA chimera"
                     /label=BCG hsp60-lysA chimera
                     /protein_id="AAY46598.1"
                     /translation="MAKTIADPDVNELLHLAPNVWPRNTTRDEVGVVCIAGIPLTQLAQ
                     EYGTPLFVIDEDDFRSRCRETAAAFGSGANVHYAAKAFLCSEVARWISEEGLCLDVCTG
                     GELAVALHASFPPERITLHGNNKSVSELTAAVKAGVGHIVVDSMTEIERLDAIAGEAGI
                     VQDVLVRLTVGVEAHTHEFISTAHEDQKFGLSVASGAAMAAVRRVFATDHLRLVGLHSH
                     IGSQIFDVDGFELAAHRVIGLLRDVVGEFGPEKTAQIATVDLGGGLGISYLPSDDPPPI
                     AELAAKLGTIVSDESTAVGLPTPKLVVEPGRAIAGPGTITLYEVGTVKDVDVSATAHRR
                     YVSVDGGMSDNIRTGLYGAQYDVRLVSRVSDAPPVPARLVGKHCESGDIIVRDTWVPTI
                     FGPAIWLRLPPPALLLFAVESLQHGRPSRCGSGARGNARLVLRRETVDDLLSLEVR"
     CDS             1877..2692
                     /codon_start=1
                     /gene="kan"
                     /product="aminoglycoside-3-phosphotransferase"
                     /label=kan
                     /note="kanamycin resistance"
                     /protein_id="AAY46599.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            1877..2692
                     /gene="kan"
                     /label=kan
     misc_feature    2979..3766
                     /note="OriE region; similar to pUC19"
     rep_origin      3024..3612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3771..5666
                     /note="OriM; mycobacterial origin of replication region; 
                     similar to pAL5000 in GenBank Accession Number M60875"
     regulatory      5873..5878
                     /regulatory_class="minus_35_signal"
                     /note="derived from Mycobacterium leprae 18 kDa promoter"
     regulatory      5896..5901
                     /regulatory_class="minus_10_signal"
                     /note="derived from Mycobacterium leprae 18 kDa promoter"
     misc_feature    5910
                     /label=transcription start site
                     /note="transcription start site"
     regulatory      5960..5966
                     /regulatory_class="ribosome_binding_site"
     CDS             5975..7303
                     /codon_start=1
                     /product="alpha antigen-rotavirus EDIM VP6 chimera"
                     /label=alpha antigen-rotavirus EDIM VP6 chimera
                     /protein_id="AAY46600.1"
                     /translation="MATDVSRKIRAWGRRLMIGTAAAVVLPGLVGLAGGAATAGAFGGP
                     MDVLYSISRTLKDARDKIVEGTLYSNVSDLIQQFNQMLVTMNGNEFQTGGIGNLPLRNW
                     NFDFGLLGTTLLNLDANYVESARTTIDYFVDFIDNVCMDEMMRESQRNGIAPQSDALRK
                     LSGVKFRRINFNNSSEYIENWNLQNRRQRTGFTFHKPNIFPYSASFTLNRSQPQHDNLM
                     GTMWLNAGSEIQVAGFDYSCAINAPANIQQFEHIVQLRRVLTTATITLLPDAERFSFPR
                     VINSADGATTWYFNPVILRPNNVEVEFLLNGQVINTYQARFGTIVARNFDTIRLSFQLM
                     RPPNMTPAVTALFPNAQPFEHHATVGLTLRIDSAICESVLADASETMLANVTSVRQEYA
                     IPVGPVFPPGMNWTDLITNYSPSREDNLQRVFTVASIRSMLVK"
     terminator      7350..7396
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      7353..7396
                     /regulatory_class="terminator"
                     /note="rrnBT1"