LOCUS       Exported                7141 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-Mgen-recA, complete sequence.
ACCESSION   JX026664
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     RecA mediates MgpB and MgpC phase and antigenic variation in 
            Mycoplasma genitalium, but plays a minor role in DNA repair
  JOURNAL   Mol. Microbiol. 85 (4), 669-683 (2012)
  PUBMED    22686427
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Burgos R, Wood GE, Young L, Glass JI, Totten PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2012) Synthetic Biology & Biooenergy, J. Craig 
            Venter Instititute, 9704 Medical Center Drive, Rockville, MD 20850, 
            USA
REFERENCE   3  (bases 1 to 7141)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Cloning vector pCAT-Mgen-recA"
                     /focus
                     /mol_type="other DNA"
                     /label=used for manipulation of Mycoplasma genitalium
                     /note="used for manipulation of Mycoplasma genitalium"
                     /db_xref="taxon:1200332"
     source          3637..4737
                     /organism="Mycoplasma genitalium"
                     /mol_type="other DNA"
                     /label=includes MG_338 5 end
                     /note="includes MG_338 5 end"
                     /db_xref="taxon:2097"
     source          5761..6805
                     /organism="Mycoplasma genitalium"
                     /mol_type="other DNA"
                     /label=includes MG_340 3 end
                     /note="includes MG_340 3 end"
                     /db_xref="taxon:2097"
     misc_feature    1..3632
                     /label=derived from Invitrogen TA Cloning Vector pCR-2.1
                     /note="derived from Invitrogen TA Cloning Vector pCR-2.1"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(941..1801)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /EC_number="3.5.2.6"
                     /label=ampR
                     /note="beta lactamase; ampicillin resistance marker"
                     /protein_id="AFM47375.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(941..1801)
                     /gene="ampR"
                     /label=ampR
     misc_feature    complement(1819..2625)
                     /gene="kanR"
                     /note="KanR; kanamycin kinase; aminoglycoside 
                     3-O-phosphotransferase; kanamycin resistance gene marker"
     gene            complement(1819..2625)
                     /gene="kanR"
                     /label=kanR
     CDS             complement(1819..2613)
                     /codon_start=1
                     /gene="aph(3)-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(2957..3385)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     3526..3542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3549..3567
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(4783..5442)
                     /codon_start=1
                     /gene="cat"
                     /product="Cat"
                     /EC_number="2.3.1.28"
                     /label=cat
                     /note="chloramphenicol O-acetyltransferase; chloramphenicol
                     resistance marker"
                     /protein_id="AFM47376.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(4783..5442)
                     /gene="cat"
                     /label=cat
     misc_feature    6811..6871
                     /label=derived from Invitrogen TA Cloning Vector pCR-2.1
                     /note="derived from Invitrogen TA Cloning Vector pCR-2.1"
     promoter        complement(6885..6903)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    6904..7141
                     /label=derived from Invitrogen TA Cloning Vector pCR-2.1
                     /note="derived from Invitrogen TA Cloning Vector pCR-2.1"
     primer_bind     complement(6921..6937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6945..6961
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6969..6999)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7014..7035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."