LOCUS       Exported                4454 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAT-3, complete sequence.
ACCESSION   AY170009
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Sarafanov AG, Kondratov RV, Zakharev VM, Prasolov VS, Kozlov,Iu.V.
  TITLE     [Gene for the human tissue activator plasminogen: regulation of 
            expression by signals, located in the 3-untranslated region]
  JOURNAL   Mol. Biol. (Mosk.) 33 (3), 413-422 (1999)
  PUBMED    10519114
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Sarafanov AG.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2002) Biochemistry, American Red Cross, Holland 
            Laboratory, 15601 Crabbs Branch Way, Rockville, MD 20855, USA
REFERENCE   3  (bases 1 to 4454)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Cloning vector pCAT-3"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /note="based on cloning vector pCAT-Control deposited in 
                     GenBank Accession Number X65321; designed for 
                     identification of eukaryotic 3 UTR expression signals"
                     /db_xref="taxon:214634"
     primer_bind     363..379
                     /label=pUC/M13 forward primer
                     /note="pUC/M13 forward primer"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      412..614
                     /regulatory_class="promoter"
                     /note="SV40 promoter"
     promoter        412..608
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      459..594
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             646..1305
                     /codon_start=1
                     /gene="CAT"
                     /product="chloramphenicol transferase"
                     /label=CAT
                     /protein_id="AAN74640.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            646..1305
                     /gene="CAT"
                     /label=CAT
     intron          1471..1536
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1666..1686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1695..1810
                     /note="multiple cloning site; contains BpiI, SacI, BstXI, 
                     NotI, XbaI, SpeI, BamHI, XmaI/SmaI, MulI, EcoRV, HindIII, 
                     ClaI, SalI/AccI, XhoI, DraII/ApaI, KpnI, and BclI 
                     restriction sites; XbaI, HindIII, SalI/AccI sites are not 
                     unique"
     primer_bind     1724..1740
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1768..1784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1811..1946
                     /label=partial SV40 polyadenylation region
                     /note="partial SV40 polyadenylation region"
     polyA_signal    1811..1945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1947..2183
                     /regulatory_class="enhancer"
                     /note="SV40 enhancer"
     primer_bind     complement(2235..2251)
                     /label=pUC/M13 reverse primer
                     /note="pUC/M13 reverse primer"
     protein_bind    2259..2275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2283..2313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2328..2349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2637..3223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3394..4254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AAN74641.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3394..4254)
                     /gene="bla"
                     /label=bla
     promoter        complement(4255..4359)
                     /gene="bla"
                     /label=AmpR promoter