LOCUS       Exported                7967 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning/transgenesis vector pCaSpeR5, complete sequence.
ACCESSION   EF090407
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
  TITLE     CaSpeR5, a family of Drosophila transgenesis and shuttle vectors 
            with improved multiple cloning sites
  JOURNAL   BioTechniques 42 (2), 164 (2007)
  PUBMED    17373479
REFERENCE   2  (bases 1 to 7967)
  AUTHORS   Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech 
            Dr., Evanston, IL 60091, USA
REFERENCE   3  (bases 1 to 7967)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Cloning/transgenesis vector pCaSpeR5"
                     /mol_type="other DNA"
                     /db_xref="taxon:412954"
     misc_feature    1..233
                     /label=P element 3 end
                     /note="P element 3 end"
     promoter        1036..1140
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1141..2001
                     /codon_start=1
                     /product="ampicillin resistance"
                     /label=ampicillin resistance
                     /note="drug resistance"
                     /protein_id="ABK79310.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2172..2760
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2995..3580
                     /label=P element 5 end
     misc_feature    7719..7967
                     /label=pCasper5 multiple cloning site
                     /note="pCasper5 multiple cloning site"