LOCUS       Exported                3048 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCASP, complete sequence.
ACCESSION   EF179157
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Secreting Spider Silk in Salmonella
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Widmaier DM, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2006) Pharmaceutical Chemistry, UCSF, 1700 4th 
            Street, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 3048)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Cloning vector pCASP"
                     /lab_host="Salmonella typhimurium"
                     /mol_type="other DNA"
                     /db_xref="taxon:418929"
     regulatory      7..170
                     /regulatory_class="promoter"
                     /note="sicA promoter; psicA"
     CDS             171..563
                     /codon_start=1
                     /gene="SicP"
                     /product="SicP"
                     /label=SicP
                     /note="secretion chaperone"
                     /protein_id="ABN48557.1"
                     /translation="MQAHQDIIANIGEKLGLPLTFDDNNQCLLLLDSDIFTSIEAKDDI
                     WLLNGMIIPLSPVCGDSIWRQIMVINGELAANNEGTLAYIDAAETLLLIHAITDLTNTY
                     HIISQLESFVNQQEALKNILQEYAKV"
     gene            171..563
                     /gene="SicP"
                     /label=SicP
     gene            550..1078
                     /gene="SptP Tag"
                     /label=SptP Tag
                     /note="type III secretion tag"
     CDS             1054..1074
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      1146..1232
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1397..1985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2073..2167
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2188..2847)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2848..2950)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"