LOCUS       Exported               10131 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCamE, complete sequence.
ACCESSION   JX841315
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Constructing a novel plant expression vector (pCamE) with CaMV 35S 
            promoter and fifteen restriction endonuclease sites in the 
            expression cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Ma Z, Wu L, Wang X, Li X, Li Y, Wang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-2012) Crop Genetics and Breeding Department, 
            Agricultural University of Hebei, South of Jianshe Street 215, 
            Baoding, Hebei 071000, P.R. China
REFERENCE   3  (bases 1 to 10131)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: DNASTAR v. 7.10
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Binary vector pCamE"
                     /mol_type="other DNA"
                     /PCR_primers="fwd_name: prof, fwd_seq: 
                     caagcttaattaagagctcgcatgccctttcag, fwd_name: terf, fwd_seq:
                     ggtaccgggccctgatctcgaggagctagctcgaatttcccc, rev_name: pror,
                     rev_seq: gtcgacgaccggtagcgctagagtccccg, rev_name: terr, 
                     rev_seq: gaattcgaattaattcccgatctagtaacatagatg"
                     /db_xref="taxon:1337424"
     primer_bind     complement(9..25)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..49
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(57..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    102..123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        314..992
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     regulatory      570..1024
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     CDS             1058..2083
                     /codon_start=1
                     /gene="hptII"
                     /product="hygromycin phosphotransferase"
                     /label=hptII
                     /protein_id="AGN03892.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     gene            1058..2083
                     /gene="hptII"
                     /label=hptII
     3UTR           2101..2309
                     /note="CaMV; polyA signal"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2402
                     /label=left border
                     /note="left border"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aadI"
                     /product="aminoglycoside phosphotransferase"
                     /label=aadI
                     /protein_id="AGN03893.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2825..3619
                     /gene="aadI"
                     /label=aadI
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3911..4191
                     /label=pBR322
                     /note="pBR322"
     misc_feature    4331..4591
                     /label=pBR322 bom
                     /note="pBR322 bom"
     misc_feature    4480..4620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      4964..5158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5001..6001
     CDS             complement(5224..6291)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    6594..7594
                     /label=pVS1 STA
                     /note="pVS1 STA"
     CDS             complement(6725..7354)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8636..8661
                     /label=right border
                     /note="right border"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      8907..9781
                     /regulatory_class="promoter"
                     /note="CaMV 35S"
     promoter        9429..9774
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    9798..9830
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     regulatory      9851..10115
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      9861..10113
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"