LOCUS       Exported               11375 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Binary vector pCAMBIA1300-TMT-2S, complete sequence.
ACCESSION   FJ362600
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Shi XG, Zhuang WJ.
  TITLE     Binary vector pCAMBIA1300-TMT-2S for making gamma-tocopherol 
            methyltransferase over expression in seed
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Zhuang WJ, Shi XG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2008) Crop Science Department, Oil Crop 
            Institution, Fujian Agriculture, Jinshan, Fuzhou, Fujian 350002, 
            P.R. China
REFERENCE   3  (bases 1 to 11375)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Binary vector pCAMBIA1300-TMT-2S"
                     /mol_type="other DNA"
                     /db_xref="taxon:571925"
     regulatory      8..260
                     /regulatory_class="terminator"
                     /note="NOS"
     terminator      8..260
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(277..1323)
                     /codon_start=1
                     /gene="VTE4"
                     /product="gamma-tocopherol methyltransferase"
                     /label=VTE4
                     /note="derived from Arabidopsis thaliana"
                     /protein_id="ACJ54270.1"
                     /translation="MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTR
                     GNVAVAAAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEA
                     QIRMIEESLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAK
                     RANDLAAAQSLSHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAA
                     PGGRIIIVTWCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQD
                     IKCADWSENVAPFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFG
                     IITCQKPL"
     gene            complement(277..1323)
                     /gene="VTE4"
                     /label=VTE4
     regulatory      1341..2501
                     /regulatory_class="promoter"
                     /note="Brassica napus 2S seed storage protein promoter"
     primer_bind     complement(2508..2524)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2727..2751
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2746..2771
                     /label=right border T-DNA repeat
                     /note="right border T-DNA repeat"
     CDS             4051..4680
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5114..6181
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6247..6441
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6785..6925
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7111..7699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7786..8580)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=aminoglycoside phosphotransferase
                     /protein_id="ACJ54268.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9005..9030
                     /label=left border C58 T-DNA repeat
                     /note="left border C58 T-DNA repeat"
     misc_feature    9005..9029
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    9097..9305
                     /label=CaMV polyA signal
                     /note="CaMV polyA signal"
     polyA_signal    9107..9281
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9321..10346)
                     /codon_start=1
                     /product="hygromycin phosphotransferase"
                     /label=hygromycin phosphotransferase
                     /note="hptII; confers hygromycin resistance"
                     /protein_id="ACJ54269.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     regulatory      10382..11162
                     /regulatory_class="promoter"
                     /note="CaMV 35S promoter"
     promoter        complement(10414..11091)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    11282..11303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11318..11348
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11356..11372
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."