LOCUS       Exported                8006 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Plant transformation vector pCAM-NOS, complete sequence.
ACCESSION   JQ733907
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.
  TITLE     Evaluation of four phloem-specific promoters in vegetative tissues 
            of transgenic citrus plants
  JOURNAL   Tree Physiol. 32 (1), 83-93 (2012)
  PUBMED    22228816
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Dutt M, Ananthakrishnan G, Jaromin M, Brlansky R, Grosser J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-FEB-2012) Citrus Research and Education Center, 
            University of Florida, 700 Experiment Station Road, Lake Alfred, FL 
            33850, USA
REFERENCE   3  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Plant transformation vector pCAM-NOS"
                     /mol_type="other DNA"
                     /db_xref="taxon:1194220"
     regulatory      65..371
                     /regulatory_class="promoter"
                     /note="NOS"
     promoter        148..327
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        180..363
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             375..1172
                     /codon_start=1
                     /product="NPTII"
                     /label=NPTII
                     /protein_id="AFM76957.1"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      1210..1413
                     /regulatory_class="terminator"
                     /note="NOS"
     polyA_signal    1229..1403
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1481..1505
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1930..2724
                     /codon_start=1
                     /product="AadA"
                     /label=AadA
                     /note="kanamycin resistance"
                     /protein_id="AFM76956.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2811..3399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3585..3725
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      4069..4263
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4329..5396)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(5830..6459)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     rep_origin      6209..7209
     misc_feature    7759..7783
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     7986..8002
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8006..56
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"