LOCUS       Exported                6738 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAhIFNGSTm, complete sequence.
ACCESSION   LT727221
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6738)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Mammalian expression vector pCAhIFNGSTm"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4482."
                     /db_xref="taxon:1944900"
     CDS             69..137
                     /codon_start=1
                     /note="unnamed protein product; hIFNG signal sequence"
                     /protein_id="SJL87797.1"
                     /translation="MKYTSYILAFQLCIVLGSLGCYC"
     CDS             149..1211
                     /codon_start=1
                     /note="unnamed protein product; mature hST6GalI mutant"
                     /protein_id="SJL87798.1"
                     /translation="GRHGV*FPVCILKQHPGPPQGPPDPRQSQRPSQGQTRGLLPGVEQ
                     GQLFQKPYP*AAKDLEELPKHEQVQSVLQGARTRHQVQCRGPALPPPGPCECIHGRGHR
                     FSLQYL*MGGLSAQGEH*DQGWALGQVCCCVVSGISEVLPTRQRNR*S*RSPEV*WGTH
                     SQLPTRCGHKNYHSPDELSVGYHREALPQRQFVQ*RNPNCMGPICIPLRYPKVVPESGL
                     *FL*QLQDLS*AAPQSALLHPQAPDALGAMGHSSRNLPRRDSAKPPILWDAWYHHHDDA
                     V*PGGYL*VPPIQAQD*RVLLLPEVLR*CLHDGCLPPAAL*EEFGEASQPGHR*GHLPA
                     WKSHTAWLPDHSLL"
     regulatory      150..159
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      2028..2119
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      2095..2150
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2120..2476
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     primer_bind     complement(2511..2527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2535..2551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(2554..2636)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(2559..2589)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2603..2624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2683..2878
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2729..2864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2778..2853
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2884..3018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2884..2958)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2884..2958
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(3073..3474)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3256..3844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4015..4875)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87799.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4876..4980)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5011..5390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5011..5388
                     /regulatory_class="promoter"
                     /note="hCMV-IE enhancer"
     promoter        5392..5669
                     /label=chicken beta-actin promoter
     regulatory      5394..6735
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5670..6687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5763..6687
                     /label=intron
                     /note="intron"