LOCUS       Exported                6430 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGSMtCAT, complete sequence.
ACCESSION   LT727344
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6430)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Mammalian expression vector pCAGGSMtCAT"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3482."
                     /db_xref="taxon:1944890"
     CDS             40..111
                     /codon_start=1
                     /note="unnamed protein product; MTS of hMnSOD"
                     /protein_id="SJL88166.1"
                     /translation="MLSRAVCGTSRQLAPVLGYLGSRQ"
     CDS             112..478
                     /codon_start=1
                     /note="unnamed protein product; rCATf"
                     /protein_id="SJL88167.1"
                     /translation="MADSRDPASDQMKQWKEQRAPQKPDVLTTGGGNPIGDKLNIMTAG
                     PRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITRYSKAKVFEHIG
                     KRTPIAVRFSTVAGESGS"
     CDS             479..1647
                     /codon_start=1
                     /note="unnamed protein product; hCATf"
                     /protein_id="SJL88168.1"
                     /translation="LTQFGTLVGLQ*NFTQKMVTGISLEITPPFSSSGIPYCFHLLSTA
                     KREILRHI*RIRTWSGTSGAYVLSLCIRFLSCSVIGGFQMDIAT*MDMDHILSSWLMQM
                     GRQFIANSIIRLTRASKTFLLKMRRDFPRKILTMASGIFLTPLPQESTPPGLFTSRS*H
                     LIRQKLFHLIHSISPRFGLTRTTLSSQLVNWS*TGIQLITLLRLNR*PSTQATCHLALR
                     PVLTKCFRAAFLPILTLTAIAWDPIIFIYL*TVPTVLEWPTTNVTARCACRTIRVVLQI
                     TTPTALVLRNNSLLPWSTASNILEKCGDSTLPMMITLLRCGHSM*TC*MRNRGNVCVRT
                     LPAT*RMHKFSSRRKRSRTSLRSTLTTGATSRLFWTSTMLRSLRMRFTP"
     regulatory      1720..1811
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      1787..1842
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1812..2168
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     primer_bind     complement(2203..2219)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2227..2243
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(2246..2328)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(2251..2281)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2295..2316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2375..2570
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2421..2556
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2470..2545
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2576..2710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2576..2650)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2576..2650
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(2765..3166)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3707..4567)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88169.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4568..4672)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4703..5082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4703..5080
                     /regulatory_class="promoter"
                     /note="hCMV enhancer"
     promoter        5084..5361
                     /label=chicken beta-actin promoter
     regulatory      5086..6427
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5362..6379
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5455..6379
                     /label=intron AG promoter
                     /note="intron AG promoter"