LOCUS       Exported                6345 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGShMnSOD, complete sequence.
ACCESSION   LT727340
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6345)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Mammalian expression vector pCAGGShMnSOD"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3451."
                     /db_xref="taxon:1944872"
     misc_feature    6..39
                     /label=5UTR
                     /note="5UTR"
     CDS             40..708
                     /codon_start=1
                     /note="unnamed protein product; hMnSOD"
                     /protein_id="SJL88157.1"
                     /translation="MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQ
                     IMQLHHSKHHAAYVNNLNVTEEKYQEALAKGDVTAQIALQPALKFNGGGHINHSIFWTN
                     LSPNGGGEPKGELLEAIKRDFGSFDKFKEKLTAASVGVQGSGWGWLGFNKQRGHLQIAA
                     CPNQDPLQGTTGLIPLLGIDVWEHAYYLQYKNVRPDYLKAIWNVINWENVTERYMACKK
                     "
     misc_feature    709..801
                     /label=3UTR
                     /note="3UTR"
     regulatory      887..978
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_site      954..1009
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    979..1335
                     /label=3FR
                     /note="3FR"
     polyA_signal    1359..1493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1359..1433)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1359..1433
                     /regulatory_class="terminator"
                     /note="polyA"
     misc_feature    1489..2234
                     /label=PY
                     /note="PY"
     rep_origin      2122..2234
                     /label=PY-O
                     /note="PY-O"
     rep_origin      2336..2471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2385..2460
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2491..2625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2491..2565)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2491..2565
                     /regulatory_class="terminator"
                     /note="polyA"
     rep_origin      complement(2680..3081)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3622..4482)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88158.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4483..4587)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4618..4997
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4618..4995
                     /label=hCMV
                     /note="hCMV"
     promoter        4999..5276
                     /label=chicken beta-actin promoter
     regulatory      5001..6342
                     /regulatory_class="promoter"
                     /note="AG"
     intron          5277..6294
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5370..6294
                     /label=intron
                     /note="intron"