LOCUS       Exported                5797 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-mCASP-3a, complete sequence.
ACCESSION   LT727404
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5797)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Mammalian expression vector pCAGGS-mCASP-3a"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3822."
                     /db_xref="taxon:1944884"
     misc_feature    complement(62..140)
                     /label=3UTR
                     /note="3UTR"
     CDS             complement(141..974)
                     /codon_start=1
                     /note="unnamed protein product; CASP3"
                     /protein_id="SJL88357.1"
                     /translation="MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEMG
                     ICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDSVS
                     KEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFIIQAC
                     RGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCSM
                     LKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFYH"
     misc_feature    complement(975..1041)
                     /label=5UTR
                     /note="5UTR"
     regulatory      1087..1178
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_site      1154..1209
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1179..1535
                     /label=3FR
                     /note="3FR"
     primer_bind     complement(1570..1586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1594..1610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1613..1695)
                     /regulatory_class="promoter"
                     /note="lac"
     promoter        complement(1618..1648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1662..1683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        1742..1937
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1788..1923
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      1837..1912
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    1943..2077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(1943..2017)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      1943..2017
                     /regulatory_class="terminator"
                     /note="polyA"
     rep_origin      complement(2130..2533)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2315..2903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3074..3934)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3935..4039)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4070..4449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4070..4447
                     /label=hCMV
                     /note="hCMV"
     promoter        4451..4728
                     /label=chicken beta-actin promoter
     regulatory      4453..5794
                     /regulatory_class="promoter"
                     /note="AG"
     intron          4729..5746
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    4822..5746
                     /label=intron
                     /note="intron"