LOCUS       Exported                6167 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-hCASP-6, complete sequence.
ACCESSION   LT727412
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6167)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Mammalian expression vector pCAGGS-hCASP-6"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3831."
                     /db_xref="taxon:1944871"
     CDS             72..953
                     /codon_start=1
                     /note="unnamed protein product; hCASP6"
                     /protein_id="SJL88373.1"
                     /translation="MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRG
                     IALIFNHERFFWHLTLPERRRTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVS
                     TVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQAC
                     RGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRET
                     VNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASM
                     LTKKLHFFPKSN"
     misc_feature    954..1403
                     /label=3UTR
                     /note="3UTR"
     regulatory      1457..1548
                     /regulatory_class="terminator"
                     /note="polyA"
     polyA_site      1524..1579
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1549..1905
                     /label=3FR
                     /note="3FR"
     primer_bind     complement(1940..1956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1964..1980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1983..2065)
                     /regulatory_class="promoter"
                     /note="lac"
     promoter        complement(1988..2018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2112..2307
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2158..2293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2207..2282
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2313..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2313..2387)
                     /regulatory_class="terminator"
                     /note="polyA"
     regulatory      2313..2387
                     /regulatory_class="terminator"
                     /note="polyA"
     rep_origin      complement(2502..2903)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2685..3273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3444..4304)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL88374.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4305..4409)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4440..4819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4440..4817
                     /label=hCMV
                     /note="hCMV"
     promoter        4821..5098
                     /label=chicken beta-actin promoter
     regulatory      4823..6164
                     /regulatory_class="promoter"
                     /note="AG"
     intron          5099..6116
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5192..6116
                     /label=intron
                     /note="intron"