LOCUS       Exported                6161 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-FLAG-hHNRPK, complete sequence.
ACCESSION   LT726997
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6161)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Mammalian expression vector pCAGGS-FLAG-hHNRPK"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5269."
                     /db_xref="taxon:1944829"
     misc_feature    13..36
                     /label=FLAG epitope
                     /note="FLAG epitope"
     CDS             13..36
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             43..1437
                     /codon_start=1
                     /note="unnamed protein product; hHNRPK"
                     /protein_id="SJL87153.1"
                     /translation="METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVEL
                     RILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKI
                     IPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVK
                     GAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRA
                     QPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYD
                     DMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFS
                     ADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLA
                     GSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQY
                     ADVEGF"
     regulatory      1447..1538
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      1514..1569
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1539..1895
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     primer_bind     complement(1930..1946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1954..1970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1973..2055)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(1978..2008)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2022..2043
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2102..2297
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2148..2283
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2197..2272
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2303..2437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2303..2377)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2303..2377
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(2492..2893)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2675..3263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3434..4294)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87154.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4295..4399)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4434..4813
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4434..4811
                     /regulatory_class="promoter"
                     /note="hCMV-IE enhancer"
     promoter        4815..5092
                     /label=chicken beta-actin promoter
     regulatory      4817..6158
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5093..6110
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5186..6110
                     /label=intron AG promoter
                     /note="intron AG promoter"