LOCUS       Exported                6079 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-EmCASP-1m3, complete sequence.
ACCESSION   LT727285
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6079)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Mammalian expression vector pCAGGS-EmCASP-1m3"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 3913."
                     /db_xref="taxon:1944810"
     misc_feature    16..54
                     /label=E-tag
                     /note="E-tag"
     CDS             61..1266
                     /codon_start=1
                     /note="unnamed protein product; mouse CASP-1, C285S-2DE 
                     mutant"
                     /protein_id="SJL87995.1"
                     /translation="ADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKLA
                     NITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEESK
                     GGHPSSSETKEEQNKEEGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLA
                     LIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEH
                     KTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQ
                     ASRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHP
                     VRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFP
                     GH"
     regulatory      1369..1460
                     /regulatory_class="terminator"
                     /note="3 UTR of beta-globin, incl. polyA"
     polyA_site      1436..1491
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1461..1817
                     /label=3 flanking region rabbit beta-globin
                     /note="3 flanking region rabbit beta-globin"
     primer_bind     complement(1852..1868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1876..1892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(1895..1977)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(1900..1930)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1944..1965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2024..2219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2070..2205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2119..2194
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2225..2359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2225..2299)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2225..2299
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(2414..2815)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2597..3185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3356..4216)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87996.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4217..4321)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4352..4731
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4352..4729
                     /regulatory_class="promoter"
                     /note="hCMV-IE enhancer"
     promoter        4733..5010
                     /label=chicken beta-actin promoter
     regulatory      4735..6076
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5011..6028
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5104..6028
                     /label=intron AG promoter
                     /note="intron AG promoter"