LOCUS       Exported                6373 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-E-hPTBdelnls, complete sequence.
ACCESSION   LT726989
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Mammalian expression vector
                     pCAGGS-E-hPTBdelnls"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5259."
                     /db_xref="taxon:1944779"
     misc_feature    11..49
                     /label=E-tag
                     /note="E-tag"
     CDS             59..1570
                     /codon_start=1
                     /note="unnamed protein product; hPTBdelnls"
                     /protein_id="SJL87136.1"
                     /translation="MVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFI
                     EMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQAVNSVQ
                     SGNLALAASAAAVDAGMAMAGQSPVLRIIVENLFYPVTLDVLHQIFSKFGTVLKIITFT
                     KNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCTLRIDFSKLTSLNVKYNNDKSRDYT
                     RPDLPSGDSQPSLDQTMAAAFGAPGIISASPYAGAGFPPTFAIPQAAGLSVPNVHGALA
                     PLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVK
                     ILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRITLSKHQNVQLPREGQEDQGLT
                     KDYGNSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVSEEDLKVLFSSNGGVVKGFKF
                     FQKDRKMALIQMGSVEEAVQALIDLHNHDLGENHHLRVSFSKSTI"
     regulatory      1658..1749
                     /regulatory_class="terminator"
                     /note="beta-globin polyA"
     polyA_site      1725..1780
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    1750..2106
                     /label=3 FR of beta-globin
                     /note="3 FR of beta-globin"
     primer_bind     complement(2141..2157)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2165..2181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(2184..2266)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(2189..2219)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2233..2254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2313..2508
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2359..2494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2408..2483
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2514..2648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2514..2588)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2514..2588
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(2703..3104)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(2886..3474)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3645..4505)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin"
                     /protein_id="SJL87137.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4506..4610)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        4641..5020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      4641..5018
                     /regulatory_class="promoter"
                     /note="hCMV-IE enhancer"
     promoter        5022..5299
                     /label=chicken beta-actin promoter
     regulatory      5024..6365
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5300..6317
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5393..6317
                     /label=intron AG promoter
                     /note="intron AG promoter"