LOCUS       Exported                6772 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCAGGS-E-hABIN-1, complete sequence.
ACCESSION   LT727094
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6772)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Mammalian expression vector pCAGGS-E-hABIN-1"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 5126."
                     /db_xref="taxon:1944769"
     misc_feature    11..49
                     /label=E-tag
                     /note="E-tag"
     regulatory      53..62
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             59..1969
                     /codon_start=1
                     /note="unnamed protein product; hABIN-1"
                     /protein_id="SJL87413.1"
                     /translation="MEGRGPYRIYDPGGSVPSGEASAAFERLVKENSRLKEKMQGIKML
                     GELLEESQMEATRLRQKAEELVKDNELLPPPSPSLGSFDPLAELTGKDSNVTASPTAPA
                     CPSDKPAPVQKPPSSGTSSEFEVVTPEEQNSPESSSHANAMALGPLPREDGNLMLHLQR
                     LETTLSVCAEEPDHGQLFTHLGRMALEFNRLASKVHKNEQRTSILQTLCEQLRKENEAL
                     KAKLDKGLEQRDQAAERLREENLELKKLLMSNGNKEGASGRPGSPKMEGTGKKAVAGQQ
                     QASVTAGKVPEVVALGAPEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQ
                     KLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQ
                     DQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQ
                     NELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKA
                     REALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPP
                     MAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGP
                     Q"
     regulatory      2057..2148
                     /regulatory_class="terminator"
                     /note="3 UTR of rabbit beta-globin, incl. polyA"
     polyA_site      2124..2179
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     misc_feature    2149..2505
                     /label=3 flanking region of rabbit beta-globin
                     /note="3 flanking region of rabbit beta-globin"
     primer_bind     complement(2540..2556)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2564..2580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      complement(2583..2665)
                     /regulatory_class="promoter"
                     /note="lac promoter"
     promoter        complement(2588..2618)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2632..2653
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2712..2907
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2758..2893
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      2807..2882
                     /label=SV40 ori
                     /note="SV40 ori"
     polyA_signal    2913..3047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(2913..2987)
                     /regulatory_class="terminator"
                     /note="SV40 polyA late"
     regulatory      2913..2987
                     /regulatory_class="terminator"
                     /note="SV40 polyA early"
     rep_origin      complement(3102..3503)
                     /direction=LEFT
                     /label=pMB1 ori
                     /note="pMB1 ori"
     rep_origin      complement(3285..3873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4044..4904)
                     /codon_start=1
                     /note="unnamed protein product; ampicillin resistance"
                     /protein_id="SJL87414.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4905..5009)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5040..5419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      5040..5417
                     /regulatory_class="promoter"
                     /note="hCMV-IE enhancer"
     promoter        5421..5698
                     /label=chicken beta-actin promoter
     regulatory      5423..6764
                     /regulatory_class="promoter"
                     /note="AG promoter"
     intron          5699..6716
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     misc_feature    5792..6716
                     /label=intron AG promoter
                     /note="intron AG promoter"