LOCUS       Exported                6371 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAG-EYFP, complete sequence.
ACCESSION   KM486813
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
            & Systems Biology, Tsinghua National Laboratory for Information 
            Science and Technology, Tsinghua University, Beijing 100084, China
REFERENCE   3  (bases 1 to 6371)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Cloning vector pCAG-EYFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1579848"
     polyA_signal    112..233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      240..695
                     /label=f1 origin
                     /note="f1 origin"
     promoter        722..826
                     /gene="bla"
                     /label=AmpR promoter
     promoter        828..1185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1036..1171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1220..2014
                     /codon_start=1
                     /product="KanR/NeoR"
                     /label=KanR/NeoR
                     /protein_id="AIZ65953.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    2246..2293
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2596..3269
                     /label=pUC origin
                     /note="pUC origin"
     rep_origin      2622..3210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3320..4975
                     /label=CAG
                     /note="CAG"
     enhancer        3380..3683
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3686..3961
                     /label=chicken beta-actin promoter
     regulatory      5058..5067
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             5064..5783
                     /codon_start=1
                     /product="EYFP"
                     /label=EYFP
                     /protein_id="AIZ65954.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    5960..6081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"