LOCUS       Exported                8223 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCa4B, complete sequence.
ACCESSION   EU420016
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Markstein M, Pitsouli C, Villalta C, Celniker SE, Perrimon N.
  TITLE     Exploiting position effects and the gypsy retrovirus insulator to 
            engineer precisely expressed transgenes
  JOURNAL   Nat. Genet. 40 (4), 476-483 (2008)
  PUBMED    18311141
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Markstein M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2008) Genetics, Harvard Medical School, 77 Avenue 
            Louis Pasteur, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Cloning vector pCa4B"
                     /mol_type="other DNA"
                     /db_xref="taxon:506679"
     misc_feature    169..235
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    236..610
                     /note="attB site cloned as SpeI/NotI fragment; derived from
                     Streptomyces lividans"
     protein_bind    313..382
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     gene            956..4144
                     /gene="mini-white"
                     /label=mini-white
                     /note="derived from Drosophila melanogaster"
     misc_feature    4785..5377
                     /label=5 P-element end
                     /note="5 P-element end"
     misc_feature    4791..5376
                     /label=P element 5 end
     rep_origin      complement(5611..6199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6370..7230)
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /protein_id="ACA42566.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7231..7335)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8137..146
                     /label=P element 3 end
                     /note="P element 3 end"
     misc_feature    8137..143
                     /label=3 P-element end
                     /note="3 P-element end"