LOCUS       Exported                8947 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCA-DEST2210, complete sequence.
ACCESSION   MG188300
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Legrand M, Bachellier-Bassi S, Lee KK, Chaudhari Y, Tournu H, 
            Arbogast L, Boyer H, Chauvel M, Cabral V, Maufrais C, Nesseir A, 
            Pelinska I, Permal E, Rossignol T, Walker LA, Zeidler U, Znaidi S, 
            Schoeters F, Majgier C, Julien RA, Ma L, Tichit M, Bouchier C, Van 
            Dyck P, Munro CA, DEnfert C.
  TITLE     Generating genomic platforms to study C. albicans pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Bachellier-Bassi S, Arbogast L, Legrand M, Boyer H, Chauvel M, 
            DEnfert C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2017) Mycology, Institut Pasteur, 25 rue du Dr 
            ROUX, Paris 75015, France
REFERENCE   3  (bases 1 to 8947)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Cloning vector pCA-DEST2210"
                     /mol_type="other DNA"
                     /note="Candida albicans integrative plasmid; Gateway 
                     destination vector; propagate in ccdB resistant Escherichia
                     coli"
                     /db_xref="taxon:2081749"
     regulatory      1..92
                     /regulatory_class="promoter"
                     /note="ampicillin promoter"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             129..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             327..989
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AUZ17305.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     gene            327..989
                     /gene="bla"
                     /label=bla
     rep_origin      1138..1766
                     /label=colE1 ori
                     /note="colE1 ori"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2207..3319
                     /regulatory_class="promoter"
                     /note="PCK1 promoter"
     CDS             3357..3446
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     misc_feature    3357..3383
                     /label=HA epitope
                     /note="HA epitope"
     misc_feature    3387..3413
                     /label=HA epitope
                     /note="HA epitope"
     misc_feature    3420..3446
                     /label=HA epitope
                     /note="HA epitope"
     regulatory      3456..3580
                     /regulatory_class="attenuator"
                     /note="Gateway recombination site attR1"
     protein_bind    3456..3580
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3594..3650
                     /regulatory_class="promoter"
                     /note="lacUV5 promoter"
     promoter        3605..3635
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3689..4348
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AUZ17306.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            3689..4348
                     /gene="cat"
                     /label=cat
     CDS             4690..4995
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             4783..4995
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB"
                     /label=ccdB
                     /note="F plasmid toxin"
                     /protein_id="AUZ17307.1"
                     /translation="MVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVI
                     GEEVADLSHRENDIKNAINLMFWGI"
     gene            4783..4995
                     /gene="ccdB"
                     /label=ccdB
     protein_bind    complement(5036..5160)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      5036..5158
                     /regulatory_class="attenuator"
                     /note="Gateway recombination site attR2"
     CDS             5462..5764
                     /codon_start=1
                     /gene="rps1"
                     /product="ribosomal protein 1"
                     /label=rps1
                     /note="RPS1"
                     /protein_id="AUZ17308.1"
                     /translation="MAVGKNKRLSKGKKGLKKKVVDPFTRKDWFDIKAPTTFENRNVGK
                     TLINRSTGLKNAADGLKGRVFEVCLADLQGSEDHSYRKIKLRVDEVQGKNLLTNFH"
     gene            5462..5764
                     /gene="rps1"
                     /label=rps1
     misc_feature    5766..5771
                     /label=StuI site for targeted integration
                     /note="StuI site for targeted integration"
     misc_feature    5771..5789
                     /label=I-SceI site for targeted integration
                     /note="I-SceI site for targeted integration"
     misc_feature    5790..5795
                     /label=StuI site for targeted integration
                     /note="StuI site for targeted integration"
     CDS             5796..6032
                     /codon_start=1
                     /gene="rps1"
                     /product="ribosomal protein 1"
                     /label=rps1
                     /note="RPS1"
                     /protein_id="AUZ17309.1"
                     /translation="HGLDFTSDKLRSLVRKWQSLVEANVTVKTSDDYVLRVFAIAFTKR
                     QPNQIKETTYAQSSKLREVRKKMIEIMQREVSN"
     gene            5796..6032
                     /gene="rps1"
                     /label=rps1
     primer_bind     complement(6320..6336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6803..7182
                     /regulatory_class="promoter"
                     /note="TEF1 promoter"
     promoter        6838..7181
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             7230..7754
                     /codon_start=1
                     /gene="sat1"
                     /product="nourseothricin resistance protein"
                     /label=sat1
                     /protein_id="AUZ17310.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY
                     RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL
                     IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY
                     WYWFSGAQDDA"
     gene            7230..7754
                     /gene="sat1"
                     /label=sat1
     primer_bind     7909..7940
                     /standard_name="SP2"
                     /label=SP2
                     /note="Illumina paired end sequencing primer 2"
     CDS             complement(7942..8238)
                     /codon_start=1
                     /product="neomycin/kanamycin resistance protein"
                     /label=neomycin/kanamycin resistance protein
                     /protein_id="AUZ17311.1"
                     /translation="ARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAP
                     AEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ"
     primer_bind     complement(8257..8289)
                     /standard_name="SP1"
                     /label=SP1
                     /note="Illumina paired end sequencing primer 1"
     promoter        complement(8312..8330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8337..8353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"