LOCUS       MG188274                8675 bp    DNA     circular SYN 10-FEB-2018
DEFINITION  Cloning vector pCA-DEST1130, complete sequence.
ACCESSION   MG188274
VERSION     MG188274.1
KEYWORDS    .
SOURCE      Cloning vector pCA-DEST1130
  ORGANISM  Cloning vector pCA-DEST1130
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Legrand,M., Bachellier-Bassi,S., Lee,K.K., Chaudhari,Y., Tournu,H.,
            Arbogast,L., Boyer,H., Chauvel,M., Cabral,V., Maufrais,C.,
            Nesseir,A., Pelinska,I., Permal,E., Rossignol,T., Walker,L.A.,
            Zeidler,U., Znaidi,S., Schoeters,F., Majgier,C., Julien,R.A.,
            Ma,L., Tichit,M., Bouchier,C., Van Dyck,P., Munro,C.A. and
            DEnfert,C.
  TITLE     Generating genomic platforms to study C. albicans pathogenesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   Bachellier-Bassi,S., Arbogast,L., Legrand,M., Boyer,H., Chauvel,M.
            and DEnfert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2017) Mycology, Institut Pasteur, 25 rue du Dr
            ROUX, Paris 75015, France
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Cloning vector pCA-DEST1130"
                     /mol_type="other DNA"
                     /db_xref="taxon:2081723"
                     /note="Candida albicans integrative plasmid; Gateway
                     destination vector; propagate in ccdB resistant
                     Escherichia coli"
     regulatory      1..92
                     /regulatory_class="promoter"
                     /note="ampicillin promoter"
     gene            327..989
                     /gene="bla"
     CDS             327..989
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AUZ17108.1"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
                     LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
                     EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
                     WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
                     HW"
     rep_origin      1138..1766
                     /note="colE1 ori"
     regulatory      2207..2497
                     /regulatory_class="promoter"
                     /note="tet operator - 7 copies"
     regulatory      2498..2869
                     /regulatory_class="promoter"
                     /note="OP4 minimal promoter"
     CDS             2846..>3391
                     /note="TAP tag"
                     /codon_start=1
                     /transl_table=11
                     /product="tandem affinity purification tag protein"
                     /protein_id="AUZ17109.1"
                     /translation="MKRRWKKNFIAVSAANRFKKISSSGALDYDIPTTASENLYFQGE
                     LKTAALAQHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNDAQAPKVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPKVDANSAGKST"
     regulatory      3395..3579
                     /regulatory_class="attenuator"
                     /note="Gateway recombination site attR1"
     regulatory      complement(3579..3628)
                     /regulatory_class="promoter"
                     /note="lacUV5 promoter"
     gene            3628..4287
                     /gene="cat"
     CDS             3628..4287
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="AUZ17110.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            4722..4934
                     /gene="ccdB"
     CDS             4722..4934
                     /gene="ccdB"
                     /note="F plasmid toxin"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB"
                     /protein_id="AUZ17111.1"
                     /translation="MVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSV
                     IGEEVADLSHRENDIKNAINLMFWGI"
     regulatory      4975..5097
                     /regulatory_class="attenuator"
                     /note="Gateway recombination site attR2"
     gene            5401..>5703
                     /gene="rps1"
     CDS             5401..>5703
                     /gene="rps1"
                     /note="RPS1"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein 1"
                     /protein_id="AUZ17112.1"
                     /translation="MAVGKNKRLSKGKKGLKKKVVDPFTRKDWFDIKAPTTFENRNVG
                     KTLINRSTGLKNAADGLKGRVFEVCLADLQGSEDHSYRKIKLRVDEVQGKNLLTNFH"
     misc_feature    5705..5710
                     /note="StuI site for targeted integration"
     misc_feature    5710..5728
                     /note="I-SceI site for targeted integration"
     misc_feature    5729..5734
                     /note="StuI site for targeted integration"
     gene            <5735..5971
                     /gene="rps1"
     CDS             <5735..5971
                     /gene="rps1"
                     /note="RPS1"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein 1"
                     /protein_id="AUZ17113.1"
                     /translation="HGLDFTSDKLRSLVRKWQSLVEANVTVKTSDDYVLRVFAIAFTK
                     RQPNQIKETTYAQSSKLREVRKKMIEIMQREVSN"
     gene            complement(6361..7173)
                     /gene="ura3"
     CDS             complement(6361..7173)
                     /gene="ura3"
                     /codon_start=1
                     /transl_table=11
                     /product="Ura3"
                     /protein_id="AUZ17114.1"
                     /translation="MTVNTKTYSERAETHASPVAQRLFRLMELKKTNLCASIDVDTTK
                     EFLELIDKLGPYVCLIKTHIDIINDFSYESTIEPLLELSRKHQFMIFEDRKFADIGNT
                     VKKQYIGGVYKISSWADITNAHGVTGNGVVEGLKQGAKETTTNQEPRGLLMLAELSSV
                     GSLAYGEYSQKTVEIAKSDKEFVIGFIAQRDMGGQEEGFDWLIMTPGVGLDDKGDGLG
                     QQYRTVDEVVSTGTDIIIVGRGLFGKGRDPDIEGKRYRNAGWNAYLKKTGQL"
     regulatory      complement(7174..7593)
                     /regulatory_class="promoter"
                     /note="URA3 promoter"
     primer_bind     7625..7656
                     /standard_name="SP2"
                     /note="Illumina paired end sequencing primer 2"
     CDS             complement(<7658..>7954)
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin/kanamycin resistance protein"
                     /protein_id="AUZ17115.1"
                     /translation="ARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLA
                     PAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ"
     primer_bind     complement(7973..8005)
                     /standard_name="SP1"
                     /note="Illumina paired end sequencing primer 1"