LOCUS       Exported                6225 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Reverse genetics vector pC-N, complete sequence.
ACCESSION   KJ648912
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG, 
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus 
            Reveals a Function for Non-structural Glycoproteins Cleavage by 
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
  PUBMED    25933376
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 
            30333, USA
REFERENCE   3  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Reverse genetics vector pC-N"
                     /mol_type="other DNA"
                     /label=derived from Crimean-Congo hemorrhagic fever virus
                     /note="derived from Crimean-Congo hemorrhagic fever virus"
                     /db_xref="taxon:1504877"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     regulatory      140..384
                     /regulatory_class="enhancer"
                     /note="CMV IE"
     regulatory      366..663
                     /regulatory_class="promoter"
                     /note="chicken beta actin promoter"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          663..1620
                     /note="chicken beta actin intron"
     CDS             1751..3199
                     /codon_start=1
                     /gene="N"
                     /product="nucleoprotein"
                     /label=N
                     /protein_id="AIN41191.1"
                     /translation="MENKIEVNNKDEMNRWFEEFKKGNGLVDTFTNSYSFCESVPNLDR
                     FVFQMASATDDAQKDSIYASALVEATKFCAPIYECAWVSSTGIVKKGLEWFEKNAGTIK
                     SWDESYTELKVDVPKIEQLTGYQQAALKWRKDIGFRVNANTAALSNKVLAEYKVPGEIV
                     MSVKEMLSDMIRRRNLILNRGGDENPRGPVSHEHVDWCREFVKGKYIMAFNPPWGDINK
                     SGRSGIALVATGLAKLAETEGKGIFDEAKKTVEALNGYLDKHKDEVDRASADSMITNLL
                     KHIAKAQELYKNSSALRAQSAQIDTAFSSYYWLYKAGVTPETFPTVSQFLFELGKQPRG
                     TKKMKKALLSTPMKWGKKLYELFADDSFQQNRIYMHPAVLTAGRISEMGVCFGTIPVAN
                     PDDAAQGSGHTKSILNLRTNTETNNPCAKTIVKLFEVQKTGFNIQDMDIVASEHLLHQS
                     LVGKQSPFQNAYNVKGNATSANII"
     gene            1751..3199
                     /gene="N"
                     /label=N
     polyA_site      3314..3369
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal"
     primer_bind     complement(3728..3744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3752..3768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3776..3806)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3821..3842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3901..4100
                     /label=SV40 ori
                     /note="SV40 ori"
     promoter        3901..4096
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3947..4082
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4102..4236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4411..5139
                     /label=MB1 ori
                     /note="MB1 ori"
     rep_origin      complement(4475..5063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5234..6094)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /protein_id="AIN41192.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(5234..6094)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(6095..6199)
                     /gene="bla"
                     /label=AmpR promoter