LOCUS       Exported                7383 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Bursa aurealis delivery vector pBursa, complete sequence.
ACCESSION   AY672109
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7383)
  AUTHORS   Bae T, Banger AK, Wallace A, Glass EM, Aslund F, Schneewind O, 
            Missiakas DM.
  TITLE     Staphylococcus aureus virulence genes identified by bursa aurealis 
            mutagenesis and nematode killing
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12312-12317 (2004)
  PUBMED    15304642
REFERENCE   2  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUL-2004) Molecular Genetics and Cell Biology, 
            Committee on Microbiology, University of Chicago, 920 E. 58th St., 
            Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2004) Molecular Genetics and Cell Biology, 
            Committee on Microbiology, University of Chicago, 920 E. 58th St., 
            Chicago, IL 60637, USA
REFERENCE   4  (bases 1 to 7383)
  AUTHORS   Bae T, Aslund F, Missiakas DM, Schneewind O.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2005) Molecular Genetics and Cell Biology, 
            Committee on Microbiology, University of Chicago, 920 E. 58th St., 
            Chicago, IL 60637, USA
REFERENCE   5  (bases 1 to 7383)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     On Oct 5, 2005 this sequence version replaced AY672109.2.
FEATURES             Location/Qualifiers
     source          1..7383
                     /organism="Bursa aurealis delivery vector pBursa"
                     /mol_type="other DNA"
                     /db_xref="taxon:291198"
     CDS             841..1008
                     /codon_start=1
                     /product="cop-6"
                     /label=cop-6
                     /protein_id="ABA61860.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILIK
                     KYAKEEYGAF"
     CDS             1089..1688
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein"
                     /label=repC
                     /protein_id="AAU04870.1"
                     /translation="MSENVIKETENKKNSRGRNWTFVLYPESAKAEWLEYLKELHIQFV
                     VSPLHDRDTDTEDRMKKEHYHILVMYEGNKSYEQIKIITEELNATIPQIAGSVKGLVRY
                     MLHMDDPNKFKYQKEDMIVYGGVDVDELLKKTTTDRYKLIKEMIEFIDEQGIVEFKSLM
                     DYAMKFKFDDWFPLLCDNSAYVIQEYIKSNRYKSDR"
     gene            1089..1688
                     /gene="repC"
                     /label=repC
     rep_origin      1219..1418
                     /label=from pE194
                     /note="from pE194"
     CDS             3034..3684
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /protein_id="AAU04871.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     gene            3034..3684
                     /gene="cat"
                     /label=cat
     mobile_element  4147..7383
                     /mobile_element_type="transposon:mariner-derived"
     CDS             complement(4512..5249)
                     /codon_start=1
                     /gene="ermB"
                     /product="rRNA methylase"
                     /label=ermB
                     /note="erythromycin resistance determinant"
                     /protein_id="AAU04869.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIPYH
                     LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
                     LSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(4512..5249)
                     /gene="ermB"
                     /label=ermB
     primer_bind     5805..5821
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5958..6346
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     rep_origin      6066..6346
                     /label=from plasmid R6K gamma
                     /note="from plasmid R6K gamma"
     CDS             complement(6574..7290)
                     /codon_start=1
                     /gene="gfp"
                     /product="green-fluorescent protein"
                     /label=gfp
                     /protein_id="AAU04872.2"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAGKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     gene            complement(6574..7290)
                     /gene="gfp"
                     /label=gfp
                     /note="from Aequorea victoria"