LOCUS       Exported                4677 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBTL-6, complete sequence.
ACCESSION   DQ091279
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Broad host range vectors for stable genomic library construction
  JOURNAL   Biotechnol. Bioeng. 94 (1), 151-158 (2006)
  PUBMED    16496398
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2005) Chemical and Biological Engineering, 
            University of Colorado @ Boulder, ECCH 111, Campus Box 424, Boulder,
            CO 80309, USA
REFERENCE   3  (bases 1 to 4677)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Cloning vector pBTL-6"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /db_xref="taxon:339418"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR"
     terminator      60..96
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..871
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             872..1534
                     /codon_start=1
                     /gene="rep"
                     /product="Rep"
                     /label=rep
                     /note="required for replication"
                     /protein_id="AAZ83207.1"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     gene            872..1534
                     /gene="rep"
                     /label=rep
                     /note="derived from pBBR1 in GenBank Accession Number 
                     X66730"
     CDS             1631..2404
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83208.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            1631..2404
                     /gene="kilA"
                     /label=kilA
     CDS             2422..3558
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83209.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            2422..3558
                     /gene="telA"
                     /label=telA
     CDS             3555..4508
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="involved in tellurite resistance"
                     /protein_id="AAZ83210.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            3555..4508
                     /gene="telB"
                     /label=telB
     regulatory      4522..4553
                     /regulatory_class="terminator"
                     /note="TonB"
     terminator      4522..4553
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     regulatory      4580..4662
                     /regulatory_class="promoter"
                     /note="lac"
     promoter        4600..4630
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4638..4654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."