LOCUS       Exported                4240 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBSPPc, complete sequence.
ACCESSION   KC544267
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P.
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis
  JOURNAL   Appl. Environ. Microbiol. 79 (8), 2836-2840 (2013)
  PUBMED    23416993
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Xu Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2013) State Key Laboratory of Microbial 
            Technology, Shandong University, South Shanda Road, Jinan, Shandong 
            250100, China
REFERENCE   3  (bases 1 to 4240)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Expression vector pBSPPc"
                     /mol_type="other DNA"
                     /db_xref="taxon:1298535"
     rep_origin      370..779
                     /label=ori region from pRO1600
                     /note="ori region from pRO1600"
     rep_origin      415..766
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             780..1613
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AGG35806.1"
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     gene            780..1613
                     /gene="rep"
                     /label=rep
     CDS             complement(1610..2206)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRRVRYLHYSLQTEKAYVYWAKAFVLWTARSHGGFRHPREMGQAEVEGFLTMLATE
                     KQVAPATHRQALNALLFLYRQVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAH
                     PPFASWRNSEEARTDRPSQQLRSLKGRPGRGGHGL"
     primer_bind     1774..1790
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1800..1818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1833..2057
                     /regulatory_class="promoter"
                     /gene="Pc"
                     /note="constitutive promoter"
     gene            1833..2057
                     /gene="Pc"
                     /label=Pc
     primer_bind     2060..2076
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2126)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2163..2181)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2202..2218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2226..2242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2250..2280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2295..2316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2589..3203)
                     /direction=LEFT
                     /label=the pMB1 derived origin of replication
                     /note="the pMB1 derived origin of replication"
     rep_origin      complement(2604..3192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3363..4223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /protein_id="AGG35807.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(3363..4223)
                     /gene="bla"
                     /label=bla
     promoter        complement(4224..88)
                     /gene="bla"
                     /label=AmpR promoter