LOCUS       Exported                3448 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBSk-BC4, complete sequence.
ACCESSION   HQ681278
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Inverted repeat constructs for RNA interference-mediated targeting 
            of movement/pre-coat protein genes of Cotton leaf curl virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Chakrabarty PK, Kalbande BB, Chavhan RL, Warade JG, Narwade AV, 
            Sable SV, Monga D, Mayee CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2010) Division of Crop Improvement, Central 
            Institute for Cotton Research, Post Bag No. 02, Shankar Nagar P.O., 
            Nagpur, Maharashtra 440010, India
REFERENCE   3  (bases 1 to 3448)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Cloning vector pBSk-BC4"
                     /mol_type="other DNA"
                     /label=sense-intron-antisense inverted repeat construct
                     /note="sense-intron-antisense inverted repeat construct"
                     /db_xref="taxon:944650"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      135..441
                     /label=F1(+) origin of replication
                     /note="F1(+) origin of replication"
     misc_feature    complement(460..1303)
                     /label=beta-galactosidase alpha-fragment
                     /note="beta-galactosidase alpha-fragment"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..1247
                     /note="MCS; multiple cloning site"
     misc_feature    659..870
                     /note="Cotton leaf curl virus isolate CLCuV coat protein 
                     beta-C4 protein fragment"
     intron          877..999
                     /note="chitinase intron 1; derived from Gossypium hirsutum"
     misc_feature    complement(1006..1217)
                     /note="Cotton leaf curl virus isolate Sirsa (Haryana) 
                     beta-C4 protein fragment"
     promoter        complement(1260..1278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1299..1315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1304..1425
                     /regulatory_class="promoter"
                     /note="lac promoter"
     protein_bind    1323..1339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1347..1377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1392..1413
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      1645..2312
                     /label=pUC origin of replication
                     /note="pUC origin of replication"
     rep_origin      complement(1701..2289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2460..3320)
                     /codon_start=1
                     /gene="Amp"
                     /product="beta-lactamase"
                     /label=Amp
                     /protein_id="ADV31347.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2460..3320)
                     /gene="Amp"
                     /label=Amp
     promoter        complement(3321..3425)
                     /gene="bla"
                     /label=AmpR promoter