LOCUS       Exported                7769 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pBSJL2, complete sequence.
ACCESSION   JQ354980
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Liu J, Xie Z, Merritt J, Qi F.
  TITLE     Establishment of a Tractable Genetic Transformation System in 
            Veillonella spp
  JOURNAL   Appl. Environ. Microbiol. 78 (9), 3488-3491 (2012)
  PUBMED    22344660
REFERENCE   2  (bases 1 to 7769)
  AUTHORS   Liu J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2012) Microbiology & Immunology, University of 
            Oklahoma Health Sciences Center, 975 NE 10th Street BRC366, Oklahoma
            City, OK 73104, USA
REFERENCE   3  (bases 1 to 7769)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Shuttle vector pBSJL2"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:1158679"
     source          1..666
                     /organism="Cloning vector pBluescript II KS"
                     /mol_type="other DNA"
                     /db_xref="taxon:262788"
     source          7642..7769
                     /organism="Cloning vector pBluescript II KS"
                     /mol_type="other DNA"
                     /db_xref="taxon:262788"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1000..2679)
                     /codon_start=1
                     /gene="rep"
                     /product="replication protein"
                     /label=rep
                     /protein_id="AFC98318.1"
                     /translation="MSDIVMTDTELYDAKVNWFKEHDYVEVEPLAFYRDLYPIGSFQVK
                     GDYSRKCGNGILLYRNEGKFKHRYIFDDLEEIKNWIGKEDIFVRGGSFIGKRVKNENAS
                     MIYALTFDLDGQGMNELEMVMQFMRNGTNIPMATYVVLSGHGLHLYYLLDKPIRATQDT
                     IRQLNKLKEGMTKLIWNRYTSTIEKPQYQYCLQGFRMVGSASKMGANYPVRAYKTGDRM
                     TIEKLVSWIPRLKDWDEYRINLTERSTVSLAKAKEIWPDWYERKIVKQEDNKWHINRAL
                     YDWWLNKIKEGATYGHRYFCIMCLAIYAIKCNISEAELKRDAYSLLDKMNELSKKGDPS
                     SQFTKMDIEAALQGYKEHFKTWGRDKIALISAIPLPINKRNYRTQEEHMKVISAIRDIV
                     KPNWREGNGRPKGCKNSNYPKAEIVKQWCLDHPNGKKIDCHRDTGLSRMTINKWWNIST
                     EELKRQKIINKIEFIRNKTIQLNETPEVLYPNTSEEIAKMRKNPDTVLELNRLGISFEQ
                     LENQHEIYVEDSVTFQELIEKEFGDDWIDALNLYLDYKMERK"
     gene            complement(1000..2679)
                     /gene="rep"
                     /label=rep
     misc_feature    complement(2680..3234)
                     /label=derived from Veillonella plasmid pVJL1
                     /note="derived from Veillonella plasmid pVJL1"
     CDS             complement(3376..5295)
                     /codon_start=1
                     /gene="tet(M)"
                     /product="tetracycline resistance protein Tet(M)"
                     /label=tet(M)
                     /protein_id="AFC98319.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTDN
                     TLLERQRGITIQTAITSFQWKNTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDG
                     VQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELHPN
                     MRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYHGSAK
                     NNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSV
                     RISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIE
                     NPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVT
                     CALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGV
                     QYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFR
                     MLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVI
                     LSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMF
                     NKIT"
     gene            complement(3376..5295)
                     /gene="tet(M)"
                     /label=tet(M)
     misc_feature    complement(5296..5508)
                     /note="promoter region of gyrA from Veillonella parvula 
                     PK1910"
     primer_bind     complement(5535..5551)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5581..5599)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5620..5636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5644..5660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5668..5698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5713..5734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    complement(6007..6626)
                     /note="ori ColE1; replication region from Escherichia coli 
                     plasmid pBluescript II KS(+)"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6781..7641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AFC98320.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(6781..7641)
                     /gene="bla"
                     /label=bla
     promoter        complement(7642..7746)
                     /gene="bla"
                     /label=AmpR promoter