LOCUS       Exported                3525 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS1, complete sequence.
ACCESSION   MH151157
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Welker DL, Hughes JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAR-2018) Biology, Utah State University, 5305 Old 
            Main Hill, Logan, UT 84322-5305, USA
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: NGEN Seqman v. 2.0
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Cloning vector pBS1"
                     /mol_type="other DNA"
                     /db_xref="taxon:2315980"
     CDS             complement(573..584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1449..2495
                     /codon_start=1
                     /gene="pheS*"
                     /product="PheS*"
                     /label=pheS*
                     /note="phenylalanine-tRNA synthetase subunit alpha*; 
                     counterselectable with para-chloro phenylalanine"
                     /protein_id="AXX39024.1"
                     /translation="MTLQAQLEALRDNTLKEIAQVATLKELNQIRVETLGKKGPITEVL
                     RGMKNLSPEERPVVGGFANEIRDLLTEAIEARKVVLENEALNAALKEESLDVTLPGKQM
                     PQGTRHILTQVMEEIEDIFLGMGYQVVEGYEVESDHYNFERMNLPKDHPARDMQDTFYI
                     SDEMLIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEGLVVD
                     KNVTMGDLKGTLEVMMKKMFGEDRKIRLRPSYFPFTEPSVEVDVSCFKCGGAGCNVCKH
                     TGWIEILGAGMVHPDVLQMSGIDPTEYSGFGFGLGPDRVAMLRYGVNDIRNFYQNDLRF
                     LNQFKVKE"
     gene            1449..2495
                     /gene="pheS*"
                     /label=pheS*
                     /note="pheS 294 AG"
     CDS             2744..3478
                     /codon_start=1
                     /gene="ermC"
                     /product="ErmC"
                     /label=ermC
                     /note="23S rRNA (adenine(2058)-N(6))-methyltransferase; 
                     erythromycin resistance"
                     /protein_id="AXX39025.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
                     DLNNISFEQFLSLFNSYKLFNK"
     gene            2744..3478
                     /gene="ermC"
                     /label=ermC