LOCUS       Exported                5718 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS-SK-QS, complete sequence.
ACCESSION   HM068612
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Potter CJ, Tasic B, Russler EV, Liang L, Luo L.
  TITLE     The Q system: a repressible binary system for transgene expression, 
            lineage tracing, and mosaic analysis
  JOURNAL   Cell 141 (3), 536-548 (2010)
  PUBMED    20434990
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Potter CJ, Tasic B, Luo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2010) Neuroscience, The Johns Hopkins School of 
            Medicine, 855 N. Wolfe Street, Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 5718)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Cloning vector pBS-SK-QS"
                     /mol_type="other DNA"
                     /db_xref="taxon:768487"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             727..3483
                     /codon_start=1
                     /gene="QS"
                     /product="QS"
                     /label=QS
                     /note="suppressor of QF transcription factor"
                     /protein_id="ADI49824.1"
                     /translation="MNTIPARHVGDVAARDPLPLPHISSSVASGMKRSFATMAMLYNDT
                     GNSNDVGAHARRPPRTLSNSRSTSAHRVPLGSWSAPNSPPRRALPHHPITASFDPDASI
                     VIAGIRGAGKSTLAIMASTAMKRKIVDLESEFHHLTGLSSSSYKKTHGPVDYGRRQIAI
                     LQNILNLHRTRAILVCSWLERDVQAMLQDFSVSNPVIYVLRDAKAIEAHLKGYDKSKVG
                     TLLDATSTVLRRCTRFEFFNVSEENLDTHSASTSPPAVPDQRHTAPYLTLKRAERHFLK
                     FLSLILPKGTIPFVESAFPLASVPVEQRRFTYALALPVSALLDKGVDIQELDVGVDAIE
                     IIVDDLATSESGPTSPLGLAPHRASEISRVVGEIRRDTVIPIILHVVFPERALYEEALL
                     ALYMTYLNHALRLAPDYLTVDLGLDSGLLGQLTTVQGTTKVIGNKQLAEVNSPRWGDPS
                     WLQAYEKAQNTGCDLVRLTRPASNPRDNTDIRQFHVAVEAVGGPRLPFIAYNTGRLGRT
                     SMCFNEILTPVTPVPTKEDAIGLRNPAHRYLQPPLTALEATQALYSAFVHDPMKLYVFG
                     ANVGYSLSPAMHNAALKACGIPHHYKPLSTANIGTLREVISDPQFAGASVGLPFKVEII
                     SLTHSLSRHAKAIGAVNTLIPVRHLTADGGIPDEVSMFNNISQAGAVRALYGENTDWIG
                     IRACLRRGLSPANAVRSTSTGLVIGAGGMARAAVYAMLQLGVKKILIFNRTFANAEKLV
                     LHFENLLVRDALPLLSTGPRSHDNTCFHIIRSRDDPLPENFKNPTMIVSCIPTHTVDNT
                     PDPEFTVPLHWLDNPTGGIVLELDYKCLTSPLLEQTRREAHRGWVAMDGLDLLPEQGFA
                     QFELFTGRRAPRRLMRREVLRAYPDDQAKSHTAQLQPRLNGIATQIS"
     gene            727..3483
                     /gene="QS"
                     /label=QS
     promoter        complement(3533..3551)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3572..3588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3596..3612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3620..3650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3665..3686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3974..4562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4733..5593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5594..5698)
                     /gene="bla"
                     /label=AmpR promoter