LOCUS       Exported                3228 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBS-KS-attB1-2-GT-SA, complete sequence.
ACCESSION   JN222910
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Venken KJ, Schulze KL, Haelterman NA, Pan H, He Y, Evans-Holm M, 
            Carlson JW, Levis RW, Spradling AC, Hoskins RA, Bellen HJ.
  TITLE     MiMIC: a highly versatile transposon insertion resource for 
            engineering Drosophila melanogaster genes
  JOURNAL   Nat. Methods 8 (9), 737-743 (2011)
  PUBMED    21985007
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Venken KJT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2011) Molecular and Human Genetics, Baylor College
            of Medicine, 1250 Moursund St., Suite N1125, Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 3228)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Cloning vector pBS-KS-attB1-2-GT-SA"
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /db_xref="taxon:1090670"
     rep_origin      complement(254..842)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1013..1873)
                     /codon_start=1
                     /product="AmpC"
                     /label=AmpC
                     /protein_id="AEP38953.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1874..1978)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2004..2459)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     2601..2617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2627..2645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_recomb     2666..2765
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    2681..2750
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    2778..2877
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     misc_recomb     complement(2917..3016)
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    2932..3001
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3041..3059)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3080..3096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3104..3120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3128..3158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3173..3194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."