LOCUS       Exported                3117 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning/transgenesis vector pBS-C5_delta_SphI, complete sequence.
ACCESSION   EF090403
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Le T, Yu M, Williams B, Goel S, Paul SM, Beitel GJ.
  TITLE     CaSpeR5, a family of Drosophila transgenesis and shuttle vectors 
            with improved multiple cloning sites
  JOURNAL   BioTechniques 42 (2), 164 (2007)
  PUBMED    17373479
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Beitel GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) BMBCB, Northwestern University, 2205 Tech 
            Dr., Evanston, IL 60091, USA
REFERENCE   3  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Cloning/transgenesis vector pBS-C5_delta_SphI"
                     /mol_type="other DNA"
                     /db_xref="taxon:412950"
     rep_origin      4..460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     601..617
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        627..645
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    661..906
                     /note="ppCasper5 multiple cloning site but SphI site 
                     deleted"
     promoter        complement(929..947)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(968..984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    992..1008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1016..1046)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1061..1082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1370..1958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2129..2989)
                     /codon_start=1
                     /product="ampicillin resistance"
                     /label=ampicillin resistance
                     /protein_id="ABK79306.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2990..3094)
                     /gene="bla"
                     /label=AmpR promoter