LOCUS       Exported                4789 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pBS-35S-YFP, complete sequence.
ACCESSION   AY189981
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Subramanian C, Kim BH, Lyssenko NN, Xu X, Johnson CH, von Arnim AG.
  TITLE     The Arabidopsis repressor of light signaling, COP1, is regulated by 
            nuclear exclusion: mutational analysis by bioluminescence resonance 
            energy transfer
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 101 (17), 6798-6802 (2004)
  PUBMED    15084749
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   von Arnim A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2002) Botany, University of Tennessee, 1406 Circle
            Drive, Knoxville, TN 37996, USA
REFERENCE   3  (bases 1 to 4789)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Expression vector pBS-35S-YFP"
                     /lab_host="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:221060"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    653..704
                     /label=polylinker
                     /note="polylinker"
     primer_bind     669..685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      705..1459
                     /regulatory_class="promoter"
                     /note="cauliflower mosaic virus dual 35S promoter"
     promoter        1116..1460
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     5UTR           1460..1602
                     /note="translational enhancer; 5UTR from tobacco etch 
                     virus"
     CDS             1603..2331
                     /codon_start=1
                     /gene="YFP"
                     /product="yellow fluorescent protein"
                     /label=YFP
                     /note="YFP reporter"
                     /protein_id="AAO48591.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKRSI"
     gene            1603..2331
                     /gene="YFP"
                     /label=YFP
     CDS             1603..2319
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      2332..2542
                     /regulatory_class="terminator"
                     /note="cauliflower mosaic virus 35S terminator"
     promoter        complement(2604..2622)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2643..2659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2667..2683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2691..2721)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2736..2757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3045..3633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3804..4664)
                     /codon_start=1
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=beta-lactamase
                     /protein_id="AAO48592.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4665..4769)
                     /gene="bla"
                     /label=AmpR promoter