LOCUS       Exported                3878 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBRcatTEM1, complete sequence.
ACCESSION   KU055483
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, 
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas, 
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Cloning vector pBRcatTEM1"
                     /mol_type="other DNA"
                     /db_xref="taxon:1776435"
     promoter        48..150
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             151..810
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyl-transferase"
                     /EC_number="2.3.1.28"
                     /label=cat
                     /note="chloramphenicol resistance marker"
                     /protein_id="ALV85903.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            151..810
                     /gene="cat"
                     /label=cat
     CDS             1449..1640
                     /codon_start=1
                     /gene="rop"
                     /product="ROP protein"
                     /label=rop
                     /note="regulator protein for plasmid replication"
                     /protein_id="ALV85904.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     gene            1449..1640
                     /gene="rop"
                     /label=rop
     misc_feature    1742..1882
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      2068..2656
     CDS             complement(2827..3687)
                     /codon_start=1
                     /gene="blaTEM1"
                     /product="TEM-1 B-lactamase"
                     /EC_number="3.5.2.6"
                     /label=blaTEM1
                     /note="periplasmic localized B-lactamase; confers 
                     resistance to B-lactam antibiotics in Gram-negative 
                     bacteria"
                     /protein_id="ALV85902.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2827..3687)
                     /gene="blaTEM1"
                     /label=blaTEM1
     promoter        complement(3694..3796)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3699..3703)
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(3735..3761)
                     /regulatory_class="promoter"
                     /gene="bla(P3)"
     gene            complement(3735..3761)
                     /gene="bla(P3)"
                     /label=bla(P3)