LOCUS       Exported               14625 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBR-XJ160, complete sequence.
ACCESSION   AY526355
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Yang Y, Liang G, Fu S, He H, Li X, Deng J, Su N, Hou Y.
  TITLE     Construction and infectious analysis of the full-length cDNA clone 
            of XJ-160 strain of Sindbis virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14625)
  AUTHORS   Yang Y, Liang G, Fu S, Li X, He H, Deng J, Hou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2004) State Key Lab of Molecular Virology & 
            Genetic Engineering, Institute of viral Disease Control and 
            Prevention, Chinese Center For Disease Control and Prevention, 100 
            Yingxin St., Xuanwu, Beijing 100052, China
REFERENCE   3  (bases 1 to 14625)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Cloning vector pBR-XJ160"
                     /focus
                     /mol_type="genomic DNA"
                     /country="China"
                     /clone="pBR-XJ160"
                     /note="produces infectious Sindbis virus strain XJ-160 upon
                     transfection into BHK-21 cells"
                     /db_xref="taxon:263359"
     source          1..11620
                     /organism="Sindbis virus"
                     /strain="XJ-160"
                     /mol_type="genomic DNA"
                     /label=corresponds to GenBank Accession Number AF103728
                     /note="corresponds to GenBank Accession Number AF103728"
                     /db_xref="taxon:11034"
     CDS             60..7517
                     /codon_start=1
                     /transl_except="(pos: 5664 .. 5666,  aa: OTHER)"
                     /product="nonstructural protein"
                     /label=nonstructural protein
                     /note="readthrough product"
                     /protein_id="AAS18435.1"
                     /translation="MERPVVNVDVDPQSPFVAQLQKSFPQFEVVAQQATPNDHANARAF
                     SHLASKLIELEVPTTATILDIGSAPARRMFSEHHYHCVCPMRSPEDPDRMMKYANKLAE
                     KANKITNKNLHEKIKDLRIVLDTPDAETPSLCFHNDVTCSTRAEYSVMQDVYINAPGTI
                     YHQAMKGVRTLYWIGFDTTQFMFSAMAGSYPAYNTNWADEKVLEARNIGLCSTKLSEGR
                     IGKLSIMRKKELKPGSRVYFSVGSTLYPEYRDSLQSWHLPSVFHLKGKQSYTCRCDTVV
                     SCEGYVVKKITISPGITGETVGYAVTNNSEGFLLCKVTDTVKGERVSFPVCTYIPATIC
                     DQMTGIMATDISPDDAQKLLVGLNQRIVINGKTNRNTNTMQNYLLPIIAQGFSKWAKER
                     KEDLDNEKKLGTRERKLIYGCLWAVRTKKVHSFYRPPGTQTSVKVPASFSAFPMSSVWT
                     TSLPMSLRQKIKLVLQPKKEEKLLQVSEELVAEAKAAFEDAQEEIRAEQLREALPPLVA
                     DKGIEAAAEVVCEVEGLQADIGAALVETPRGHVRIIPQVTDRMIGQYIVVSPTSVLKNA
                     KLTPVHPLADQVKIITHSGRTGRFAVEPYDAKVLMPAGSAVPWPEFLALSESATLVYNE
                     REFVNRKLYHIAIHGPAKNTEEEQYKVTKAELAETEYVFDVDKKRCVKKEEASGLVLSG
                     ELTNPPYHEMALEGLKTRPAVPYKVETIGVIGTPGSGKSAIIKSTVTVRDLVTSGKKEN
                     CREIETDVLRLRGMQITSRTVDSVMLNGCHKAVEVLYVDEAFACHAGTLLALIAIVRPR
                     KKVVLRGDPKQCGFFNMMQLKVHFNHPEKDICTKTFYKFISRRCTQPVTAIVSTLHYDG
                     KMKTTNPCKKSIEIDITGTTKPKPGDLVLTCFRGWVKQLQIDYAGNEVMTAAASQGLTR
                     KGVYAVRQKVNENPLYAITSEHVNVLLTRTEDRLVWKTLQGDPWIKQLTNIPKGNFQAT
                     IEDWEAEHKGIIAAINSPTPRINPFSCKTNVCWAKALEPILATAGIVLTGCQWSELFPQ
                     FVDDKPHSAIYALDVICIKFFGMDLTSGLFSKQSIPLTYHPADSARPVAHWDNSPGTRK
                     YGYDHAVAAELSRRFPVFQLAGKGTQLDLQTGRTRVVSAQCNLVPVNRNLPHALVPEYK
                     EKQPGPIKNFLNQFKHHSILVVSETKIEVPNKRIEWIAPLGIAGADKSYNLAFGFPPQA
                     RYDMVFINIGTKYRNHHFQQCEDHAATLKTLSRSALNCLNPGGTLVVKSYGYADRNSED
                     VVTALARKFVRVSAARPECVSSNTEMYLIFRQLDNSRTRQFTPHHLNCVISSVYEGTRE
                     GVGAAPSYRVKRENIADCHEEAIVNAANSLGKPGEGVCRAVYKRWPSSFMDSATETGTA
                     KLTVSQGMKVIHAVGPDFRKYPEAEALKLLQNAYHAVADLVNKHNIKSIAIPLLSTGIY
                     AAGKDRLEVSLNCLTTALDRTDADVTIYCLDKKWKERIDAVLQLKESVTELKDEDMEID
                     DELVWIHPDSCLKGRKGYSTTKGKLYSYFEGTKFHQAAKDMAEIKVLFPDDQESNEQLC
                     AYILGETMEAIREKCPVDRNPSSSPPKTLPCLCMYAMTPERVHRLRSNNVKEITVCSST
                     PLPKYKIKNVQKVQCTKVVLFNPHTPTFVPARKYVEVPESPAITPVQADTLDQPPAADG
                     IPLDVTDISLNMEDSSEGLSILDFHGSESSIFSMDSWSSGTSSLGPEDNRRQVVTVDVH
                     STQEDTPIPPPRLKKLARLAAAKQTPVALTVSNDVGSMDESLCLSFGSVSMSFGSFSDG
                     EIDEISRMKTESEPVLFGTFEPGEVNSIISSRSAVSFPPLRQRRRRRNKRTEY*LTGVG
                     GYIFSTDTGPGHLQKKSVLQNQFSEPTLERNVLEKIYAPTLDTSKEELLKFRYQMMPTE
                     ANKSRYQSRKVENQKAVTTERLLSGLRLYTSATDQPECYKITYPKPLYSSSVPASYSDP
                     KFAVAVCNNYLHENYPTVASYQITDEYDAYLDMVDGTVACLDTATFCPAKLRSYPKRHE
                     YRAPNIRSAVPSAMQNTLQNVLIAATKRNCNVTQMRELPTLDSATFNVECFKKYACNNE
                     YWEEFARKPIRITTEFVTAYVARLKGPKAAALFAKTYNLVPLQEVPMDRFVMDMKRDVK
                     VTPGTKHTEERPKVQVIQAAEPLATAYLCGIHRELVRRLTAVLLPNIHTLFDMSAEDFD
                     AIIAEHFKHGDPVLETDIASFDKSQDDAMALTGLMILEDLGVDHLLLDLIECAFGEISS
                     THLPTGTRFKFGAMMKSGMFLTLFVNTVLNVVIACRVLEERLKMSRCAAFIGDDNIIHG
                     VVSDKVMAERCATWLNMEVKIIDAIIGERPPYFCGGFILQDSVTSTACRVADPLKRLFK
                     LGKPLPADDEQDEDRRRALLDETKAWFRVGITDTLATAVATRYEVDNITPVLLALRTFA
                     QSKNSFQAIRGEIKQLYGGPK"
     CDS             7563..11300
                     /codon_start=1
                     /product="structural protein"
                     /label=structural protein
                     /protein_id="AAS18436.1"
                     /translation="MNRGFFNMLGRRPFPAPTSMWRPRRRRQAAPMPARNGLASQIQQL
                     TTAVSALVIGQAARPQQPRPRPPPRQKKQPPKQLPKPKKPKTQDKKKRQPAKPKPGKRQ
                     RMALKLEADRLFDVKNENGDVIGHALAMEGKVMKPLHVKGTIDHPVLSKLKFTKSSAYD
                     MEFAQLPVNMRSEAFTYTSEHPEGFYNWHHGAVQYSGGRFTIPRGVGGRGDSGRPIMDN
                     AGRVVAIVLGGADEGTRTALSVVTWNSKGKTIKTSPEGTEEWSAAPLVTAMCLLGNVSF
                     PCNRPPTCYTREPSRALDILEENVNHEDYDTLLDAILRCDFSGRNKRSVTGDFTLTSPY
                     LGTCPYCHHTEPCFSPIKIEQVWDEADDTTIRIQTSAQFGYDQSGATSVNKYRYMSFDQ
                     DHTVKEGQMDDIKISTSGPCRRLGHKGYFLLAKCPPGDSVTVSIVSSSSTTSCTLARKI
                     KPKFVGRERYDLPPVYGKNIPCRMYDRLKETSAGYITMHRPGPHAYTSYLEEASGKIYA
                     KPPSGKNITYECKCGDYKTGTVKTRTEITGCTAIKQCVAYKSDQTKWVFNSPDLIRHAD
                     HAAQGKLHLPFRLVPSSCKVPVAHAPSVVHGFKHISLQLDTDHLTLLTTRRLGANPEPT
                     SEWIIGKTVRNFSVGRDGLEYTWGNHDPVRVYAQESAPGDPHGWPHEIIQHYYHRHPAY
                     TILTVVSAAVAVLIGLTVAALCTCKARRECLTPYALAPNAVVPTSIALLCCIRSANAET
                     FSETMSYLWSNSQPFFWAQLCIPLAAVVILVRYCSCCLPFLVVAGVYLGKVDAYEHATT
                     IPNVPKIPYKALVERSGYAPLNLEITVVSSQVLPSTNQEYITCKFTTVVPSPKVKCCGS
                     LECQPAAHADYNCKVFGGVYPFMWGGAQCFCDSENTQMSEAYVKLSADCVTDYAQAVKV
                     HTAAMKVGLRIVYGNTTSYLDVYVNGVTPGTSKDLKVIAGPVSSSFTPFNHKVVIYRGL
                     VYNYDFPEYGAMKPGVFGDIQATSLTSRDLIASTDIRLLKPSVKNVHVPYTQAASGFEM
                     WKNNSGRPLQETAPFGCKIAVNPLRAVDCSYGNIPISIDIPNAAFIRISDAPLVSTVKC
                     EVSECTYSADFGGMATLQYVSDREGQCPVHSHSSTATLQESTVHVLEKGAVTVHFSTAS
                     PQANFIISLCGKKTTCNAECKPPADHIVSTPHKIDQEFQTAISKTSWSWLLALFGGASS
                     LLIIGLMIFTCSMLLTSTRR"
     promoter        11768..11872
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11873..12733
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12904..13492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    13678..13818
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(13920..14111)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     regulatory      14607..14625
                     /regulatory_class="promoter"
                     /note="SP6 promoter"
     promoter        14608..1
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"