LOCUS       Exported                8599 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBPL, complete sequence.
ACCESSION   KP869828
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Sharon-Gojman R, Maimon E, Leu S, Zarka A, Boussiba S.
  TITLE     Advanced Methods for Genetic Engineering of Haematococcus pluvialis 
            (Chlorophyceae, Volvocales)
  JOURNAL   Algal Res 10, 8-15 (2015)
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   Sharon-Gojman R, Leu S, Zarka A, Maimon E, Boussiba S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-FEB-2015) Microalgal Biotechnology Lab, Jacob 
            Blaustein Institutes for Desert Research, Sede Boquer Campus, 
            Ben-Gurion University, Sede-Boquer 8499000, Israel
REFERENCE   3  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Cloning vector pBPL"
                     /mol_type="other DNA"
                     /db_xref="taxon:1628830"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     101..117
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            126..5769
                     /gene="pdsMod4.1"
                     /label=pdsMod4.1
     regulatory      126..2124
                     /regulatory_class="promoter"
                     /gene="pdsMod4.1"
     mRNA            join(2085..2454,2544..2656,2762..3128,3481..3615,3909..3987,
                     4338..4687,4919..4989,5167..5769)
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1 mRNA
     5UTR           2085..2124
                     /gene="pdsMod4.1"
     CDS             join(2125..2454,2544..2656,2762..3128,3481..3615,3909..3987,
                     4338..4687,4919..4989,5167..5431)
                     /codon_start=1
                     /gene="pdsMod4.1"
                     /product="norflurazon resistant phytoene desaturase"
                     /label=pdsMod4.1
                     /protein_id="AKO71907.1"
                     /translation="MQTAMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP
                     DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR
                     DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA
                     PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFAIGLLPAIIFGQRYCEEQDELT
                     VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD
                     GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM
                     PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA
                     DMSTTCKEYADDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK
                     ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV
                     TEAIVEDWSARGVTSSAASRQPALAAAGVVAGSAAVIGAALVAASGAIAGGM"
     misc_feature    5232..5233
                     /gene="pdsMod4.1"
                     /label=L504R mutation
                     /note="L504R mutation"
     3UTR           5432..5769
                     /gene="pdsMod4.1"
     misc_feature    5770..5821
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(5789..5805)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5842..5860)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5881..5897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5905..5921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5929..5959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5974..5995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6283..6871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7042..7902)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="AKO71908.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(7042..7902)
                     /gene="amp"
                     /label=amp
     promoter        complement(7903..8007)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8034..8489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"