LOCUS       Exported               10755 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBOMB4R, complete sequence.
ACCESSION   KF790908
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia 
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
  PUBMED    24443531
REFERENCE   2  (bases 1 to 10755)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 
            59840, USA
REFERENCE   3  (bases 1 to 10755)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Cloning vector pBOMB4R"
                     /mol_type="other DNA"
                     /label=derived from Chlamydia trachomatis L2 LGV434/Bu
                     /note="derived from Chlamydia trachomatis L2 LGV434/Bu"
                     /db_xref="taxon:1452361"
     misc_feature    1..7554
                     /label=pL2
                     /note="pL2"
     regulatory      7555..7742
                     /regulatory_class="promoter"
                     /gene="rpoB"
     gene            7555..7742
                     /gene="rpoB"
                     /label=rpoB
     misc_feature    7743..7777
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        7778..7849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8034..8413
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5-AACGTT-3 (Sato et al., 1996)"
     rep_origin      8856..9530
                     /label=pUC19 origin replication
                     /note="pUC19 origin replication"
     rep_origin      8881..9468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"