LOCUS       Exported               10556 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBOMB4, complete sequence.
ACCESSION   KF790906
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia 
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
  PUBMED    24443531
REFERENCE   2  (bases 1 to 10556)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 
            59840, USA
REFERENCE   3  (bases 1 to 10556)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10556
                     /organism="Cloning vector pBOMB4"
                     /mol_type="other DNA"
                     /label=derived from Chlamydia trachomatis L2 LGV434/Bu
                     /note="derived from Chlamydia trachomatis L2 LGV434/Bu"
                     /db_xref="taxon:1452358"
     misc_feature    1..7554
                     /label=pL2
                     /note="pL2"
     misc_feature    7555..7589
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        7590..7661
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7662..8522
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="AHH02463.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8667..9340
                     /label=pUC19 origin replication
                     /note="pUC19 origin replication"
     rep_origin      8692..9275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             9815..10531
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /protein_id="AHH02462.1"
                     /translation="MSKGEALFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"