LOCUS       Exported               12003 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pBOMB4-Tet-mCherry, complete sequence.
ACCESSION   KF790910
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Expression and Targeting of Secreted Proteins from Chlamydia 
            trachomatis
  JOURNAL   J. Bacteriol. 196 (7), 1325-1334 (2014)
  PUBMED    24443531
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Bauler LD, Hackstadt T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2013) Laboratory of Intracellular Parasites, Rocky
            Mountain Laboratories, NIAID, NIH, 903 S. Fourth St., Hamilton, MT 
            59840, USA
REFERENCE   3  (bases 1 to 12003)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Cloning vector pBOMB4-Tet-mCherry"
                     /mol_type="other DNA"
                     /label=derived from Chlamydia trachomatis L2 LGV434/Bu
                     /note="derived from Chlamydia trachomatis L2 LGV434/Bu"
                     /db_xref="taxon:1452360"
     misc_feature    1..7554
                     /label=pL2
                     /note="pL2"
     CDS             complement(7567..8190)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     regulatory      8194..8293
                     /regulatory_class="promoter"
                     /gene="tet"
     gene            8194..8293
                     /gene="tet"
                     /label=tet
     promoter        8209..8264
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    8245..8263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             8282..8293
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8308..9018
                     /codon_start=1
                     /product="mCherry"
                     /label=mCherry
                     /protein_id="AHH02466.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPDAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        9037..9108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9109..9969
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="AHH02467.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10114..10787
                     /label=pUC19 origin replication
                     /note="pUC19 origin replication"
     rep_origin      10139..10722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             11262..11978
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=green fluorescent protein
                     /protein_id="AHH02468.1"
                     /translation="MSKGEALFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"